The genetic Ca2+ sensor GCaMP3 reveals multiple Ca2+ stores differentially coupled to Ca2+ entry in the human malaria parasite Plasmodium falciparum

Author(s)

Borges-Pereira, Lucas ^{[1, 2]} ; Thomas, Samantha J. ^[2] ; dos Anjos e Silva, Amanda Laizy ^[3] ; Bartlett, Paula J. ^[2] ; Thomas, Andrew P. ^[2] ; Garcia, Celia R. S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil

^[2] Rutgers State Univ, New Jersey Med Sch, Dept Pharmacol Physiol & Neurosci, Newark, NJ - USA

^[3] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Biological Chemistry; v. 295, n. 44, p. 14998-15012, OCT 30 2020.
• Web of Science Citations: 1

Abstract

Cytosolic Ca2+ regulates multiple steps in the host-cell invasion, growth, proliferation, and egress of blood-stage Plasmodium falciparum, yet our understanding of Ca2+ signaling in this endemic malaria parasite is incomplete. By using a newly generated transgenic line of P. falciparum (PfGCaMP3) that expresses constitutively the genetically encoded Ca2+ indicator GCaMP3, we have investigated the dynamics of Ca2+ release and influx elicited by inhibitors of the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase pumps, cyclopiazonic acid (CPA), and thapsigargin (Thg). Here we show that in isolated trophozoite phase parasites: (i) both CPA and Thg release Ca2+ from intracellular stores in P. falciparum parasites; (ii) Thg is able to induce Ca2+ release from an intracellular compartment insensitive to CPA; (iii) only Thg is able to activate Ca2+ influx from extracellular media, through a mechanism resembling store-operated Ca2+ entry, typical of mammalian cells; and (iv) the Thg-sensitive Ca2+ pool is unaffected by collapsing the mitochondria membrane potential with the uncoupler carbonyl cyanide m-chlorophenyl hydrazone or the release of acidic Ca2+ stores with nigericin. These data suggest the presence of two Ca2+ pools in P. falciparum with differential sensitivity to the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase pump inhibitors, and only the release of the Thg-sensitive Ca2+ store induces Ca2+ influx. Activation of the store-operated Ca2+ entry-like Ca2+ influx may be relevant for controlling processes such as parasite invasion, egress, and development mediated by kinases, phosphatases, and proteases that rely on Ca2+ levels for their activation. (AU)

• FAPESP's process: 18/07177-7 - EMU concedido no processo 2011/51295-5: image system
• Grantee: Célia Regina da Silva Garcia
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 16/13590-9 - MANUTENÇÃO DE LABORATÓRIO DE BIOLOGIA CELULAR E MOLECULAR DE Plasmodium falciparum: MANUTENÇÃO DE CEPAS DE Plasmodium E ORGANIZAÇÃO DO LABORATÓRIO
• Grantee: Amanda Laizy do Anjos e Silva
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 16/14411-0 - PfGCaMP3 as a new tool to study calcium signaling in the Human Malaria parasite Plasmodium falciparum
• Grantee: Lucas Borges Pereira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/08684-7 - Decoding Plasmodium signaling at molecular level as a new tool to the development of new antimalarials
• Grantee: Célia Regina da Silva Garcia
• Support Opportunities: Research Projects - Thematic Grants