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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Local and systemic immune mediators of Morada Nova lambs with divergentHaemonchus contortusresistance phenotypes

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Barbosa Toscano, Joao Henrique [1] ; Okino, Cintia Hiromi [2] ; Barbosa dos Santos, Isabella [1] ; Giraldelo, Luciana Aparecida [3] ; Borsch von Haehling, Marei [1] ; Novita Esteves, Sergio [2] ; Bassetto, Cesar Cristiano [4] ; Talamini do Amarante, Alessandro Francisco [4] ; de Souza Chagas, Ana Carolina [2]
Total Authors: 9
[1] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Jaboticabal - Brazil
[2] Embrapa Pecuaria Sudeste, Sao Carlos - Brazil
[3] Ctr Univ Cent Paulista, Sao Carlos - Brazil
[4] Univ Estadual Paulista UNESP, Inst Biociencias, Botucatu, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PARASITE IMMUNOLOGY; v. 42, n. 12 DEC 2020.
Web of Science Citations: 0

Aims Local and systemic immune mediators of Morada Nova lambs with divergentHaemonchus contortusresistance phenotypes were evaluated. Methods and Results Lambs were ranked through faecal egg counts (FEC) after two parasitic challenges with 4,000H.contortusL(3). After the second challenge, the lambs underwent a third artificial infection and were euthanized 7 days later. Immune-related genes were quantified locally in abomasal mucosa and lymph nodes (CD4,IFN gamma,IL4,IL5,IL13,IL2RAandMS4A2) and systemically in the whole blood (IL4andIL13). Anti-H. contortusIgG and IgA antibodies and eosinophils and mast cells counts were also investigated. Resistant animals presented higher systemic IgG and IgA titres, both negatively correlated with FEC. Susceptible animals had higher blood levels ofIL4transcripts. At the local level, resistant lambs had higher eosinophils counts and superiorMS4A2levels in abomasal fundic mucosa, besides higher IgA levels in abomasal mucus, while susceptible lamb had superiorIL4expression in abomasal lymph nodes. Conclusion These data indicate that resistant lambs had an immune response mediated by antibody-mediated cytotoxicity. Also, the systemic humoral profile, particularly IgA isotype, seems to be a good resistance marker for Morada Nova sheep, as we found differences between groups even when FEC did not differ. (AU)

FAPESP's process: 17/01626-1 - Genetic and immune responses characterization associated with the phenotype of parasite resistance in a sheep flock of Morada Nova
Grantee:Ana Carolina de Souza Chagas
Support type: Regular Research Grants
FAPESP's process: 17/24289-0 - Morada Nova newborns: care after birth, evaluation of levels of infection by gastrointestinal nematodes and its impact on weight gain
Grantee:Luciana Aparecida Giraldelo
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/00373-2 - Characterization of local immune responses associated with the parasitic resistance phenotype in morada nova lambs
Grantee:João Henrique Barbosa Toscano
Support type: Scholarships in Brazil - Master