Development of microsatellite markers for the predatory mite Phytoseiulus macropilis and cross-amplification in three other species of phytoseiid mites

Author(s)

Queiroz, Maria Cristina Vitelli ^[1] ; de Oliveira, Fernanda Ancelmo ^[2] ; de Souza, Anete Pereira ^[2] ; Sato, Mario Eidi ^[1]

Affiliation

^[1] APTA, Inst Biol, Lab Acarol, Rodovia Heitor Penteado Km 3-5, Caixa Postal 70, BR-13001970 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Dept Plant Biol, Cidade Univ Zeferino Vaz, CP 6109, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Experimental and Applied Acarology; v. 83, n. 1 NOV 2020.
• Web of Science Citations: 0

Abstract

Phytoseiid mites are efficient predators of mites and small pest insects. Understanding the dispersion and distribution pattern of phytoseiid mites is essential to promote the conservation of these natural enemies and support their use in biological control. Population genetic studies using molecular markers such as microsatellites have proved to be extremely informative to address questions about population structure and dispersion patterns of predatory mites. The objective of this work was to develop specific microsatellite markers for the predatory mite Phytoseiulus macropilis, aiming at improving field dispersion studies. For this purpose, the genomic DNA was extracted from the whole body of a pool of 260 adult females and used to build the genomic microsatellites-enriched library, using biotinylated probes (CT)(8) and (GT)(8). In total 26 pairs of primers were synthesized and screened across 30 adult females of P. macropilis for characterization. Seven loci were polymorphic, revealing from two to six alleles per locus. Cross amplifications were successfully obtained in the species Phytoseiulus persimilis, Amblyseius swirskii and Proprioseiopsis sp. The molecular markers obtained are the first developed for P. macropilis-they are effective for the detection and quantification of genetic variation, and show high transferability, thus can be used in genetic and molecular studies of this and other species of the same genus and also of close genera. (AU)

• FAPESP's process: 16/06919-4 - Acaricide resistance in phytophagous (Tetranychus urticae) and predaceous (Phytoseiulus macropilis) mites and strategies for the management of pest mites with the use of predaceous mites (Phytoseiidae) in ornamentals and citrus
• Grantee: Mário Eidi Sato
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/18527-9 - Phylogenetic relationships and identification of markers for apomixis and species-specific Plicatula group in polyploid genomes of Paspalum
• Grantee: Fernanda Ancelmo de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/50334-3 - Institutional research development plan (PDIp): modernization and adequacy of strategic multi-user units of the Instituto Biológico
• Grantee: Ana Eugênia de Carvalho Campos
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 15/26288-6 - Biochemistry and molecular characterization of resistance of Phytoseiulus macropilis (Banks) (Acari: Phytoseiidae) to pyrethroid, release in the field and monitoring of predators with molecular markers
• Grantee: Maria Cristina Vitelli Queiroz
• Support Opportunities: Scholarships in Brazil - Doctorate