| Full text | |
| Author(s): |
Fiorin, Gabriel L.
[1, 2, 3]
;
Sanchez-Vallet, Andrea
[3, 4]
;
Thomma, Bart P. H. J.
[3]
;
Pereira, Goncalo A. G.
[2]
;
Teixeira, Paulo J. P. L.
[5]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Grad Program Genet & Mol Biol, Campinas - Brazil
[2] Univ Estadual Campinas, Lab Genom & Expressao, Campinas - Brazil
[3] Wageningen Univ & Res, Lab Phytopathol, Wageningen - Netherlands
[4] Swiss Fed Inst Technol, Inst Integrat Biol, Plant Pathol, Zurich - Switzerland
[5] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Dept Ciencias Biol, Lab Genet & Imunol Plantas, Piracicaba - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | BIO-PROTOCOL; v. 10, n. 8 APR 20 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Plants recognize a wide variety of microbial molecules to detect and respond to potential invaders. Recognition of Microbe-Associated Molecular Patterns (MAMPs) by cell surface receptors initiate a cascade of biochemical responses that include, among others, ion fluxes across the plasma membrane. A consequence of such event is a decrease in the concentration of extracellular H+ ions, which can be experimentally detected in plant cell suspensions as a shift in the pH of the medium. Thus, similarly to reactive oxygen species (ROS) accumulation, phosphorylation of MAP kinases and induction of defense-related genes, MAMP-induced medium alkalinization can be used as a proxy for the activation of plant immune responses. Here, we describe a detailed protocol for the measurement of medium alkalinization of tobacco BY-2 cell suspensions upon treatment with two different MAMPs: chitohexamers derived from fungal cell walls (NAG6; N-acetylglucosamine) and the flagellin epitope flg22, found in the bacterial flagellum. This method provides a reliable and fast platform to access MAMP-Triggered Immunity (MTI) in tobacco cell suspensions and can be easily adapted to other plant species as well as to other MAMPs. (AU) | |
| FAPESP's process: | 13/09878-9 - Functional characterization of a chitinase-like protein of Moniliophthora perniciosa with a potential role in fungal pathogenicity during the development of the witches' broom disease of cacao |
| Grantee: | Gabriel Lorencini Fiorin |
| Support Opportunities: | Scholarships abroad - Research Internship - Scientific Initiation |
| FAPESP's process: | 09/50119-9 - Integrated and comparative study of three fungal diseases of cacao: witches' broom, frosty pod rot and brown-rot, aiming at understanding the pathogenic mechanisms for the development of control strategies |
| Grantee: | Gonçalo Amarante Guimarães Pereira |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 11/23315-1 - Functional characterization of a chitinase-like protein of Moniliophthora perniciosa with a potential role in fungal pathogenicity during the development of the witches' broom disease of cacao |
| Grantee: | Gabriel Lorencini Fiorin |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 14/06181-0 - Development of a CRISPR/Cas9-based methodology for genome engineering in the fungus Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao |
| Grantee: | Gabriel Lorencini Fiorin |
| Support Opportunities: | Scholarships in Brazil - Master |