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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genomic Analysis of SXT/R391 Integrative Conjugative Elements From Proteus mirabilis Isolated in Brazil

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Author(s):
Sato, Juliana L. [1] ; Fonseca, Marina R. B. [1] ; Cerdeira, Louise T. [1, 2] ; Tognim, Maria C. B. [3] ; Sincero, Thais C. M. [4] ; Noronha do Amaral, Mario C. [5] ; Lincopan, Nilton [1] ; Galhardo, Rodrigo S. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[2] Monash Univ, Dept Infect Dis, Cent Clin Sch, Melbourne, Vic - Australia
[3] Univ Estadual Maringa, Dept Basic Hlth Sci, Maringa, Parana - Brazil
[4] Univ Fed Santa Catarina, Dept Clin Anal, Hlth Sci Ctr, Florianopolis, SC - Brazil
[5] Univ Sao Paulo, Dept Clin Anal, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: FRONTIERS IN MICROBIOLOGY; v. 11, OCT 20 2020.
Web of Science Citations: 0
Abstract

Integrative conjugative elements (ICEs) are widespread in many bacterial species, often carrying antibiotic resistance determinants. In the present work, we screened a collection of Proteus mirabilis clinical isolates for the presence of type 1 SXT/R391 ICEs. Among the 76 isolates analyzed, 5 of them carry such elements. The complete sequences of these elements were obtained. One of the isolates carried the CMY-2 beta-lactamase gene in a transposon and is nearly identical to the element ICEPmiJpn1 previously described in Japan, and later shown to be present in other parts of the world, indicating global spread of this element. Nevertheless, the Brazilian isolate carrying ICEPmiJpn1 is not clonally related to the other lineages carrying the same element around the world. The other ICEs identified in this work do not carry known antibiotic resistance markers and are diverse in variable gene content and size, suggesting that these elements may be responsible for the acquisition of other advantageous traits by bacteria. Some sequences carried by these elements in Brazilian strains were not previously found in other SXT/R391 variants. (AU)

FAPESP's process: 19/19435-3 - The role of DNA damage and mitochondrial function in vascular, immune and neurological ageing (DNA MoVINg)
Grantee:Carlos Frederico Martins Menck
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/11348-3 - Characterization of mutator phenotypes in Proteus mirabilis isolates
Grantee:Marina Rocha Borges da Fonseca
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/23872-7 - Integrative conjugative elements of SXT/R391 family in Proteus mirabilis and its correlation with resistance, mutagenesis and conjugation
Grantee:Juliana Lumi Sato
Support Opportunities: Scholarships in Brazil - Master