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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Exercise intolerance establishment in pulmonary hypertension: Preventive effect of aerobic exercise training

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Vieira, J. S. [1] ; Cunha, T. F. [1] ; Paixao, N. A. [1] ; Dourado, P. M. [2] ; Carrascoza, L. S. [1] ; Bacurau, A. V. N. [1] ; Brum, P. C. [1]
Total Authors: 7
[1] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil
[2] Univ Sao Paulo, Med Sch, Heart Inst, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Life Sciences; v. 261, NOV 15 2020.
Web of Science Citations: 0

Aims: 1) Characterize the progression of exercise intolerance in monocrotaline-induced pulmonary hypertension (PH) in mice and 2) evaluate the therapeutic effect of aerobic exercise training (AET) on counteracting skeletal and cardiac dysfunction in PH. Main methods: Wild type C57BL6/J mice were studied in two different time points: 2 months and 4 months. Exercise tolerance was evaluated by graded treadmill exercise test. The AET was performed in the last month of treatment of 4 months' time point. Cardiac function was evaluated by echocardiography. Skeletal muscle cross-sectional area was assessed by immunofluorescence. The diameter of cardiomyocytes and pulmonary edema were quantified by staining with hematoxylin-eosin. The variables were compared among the groups by two-way ANOVA or non-paired Student's t-test. Significance level was set at p < 0.05. Key findings: After 2 months of MCT treatment, mice presented pulmonary edema, right cardiac dysfunction and left ventricle hypertrophy. After 4 months of MCT treatment, mice showed pulmonary edema, right and left cardiac dysfunction and remodeling associated with exercise intolerance and skeletal muscle atrophy. AET was able to reverse cardiac left ventricle dysfunction and remodeling, prevent exercise intolerance and skeletal muscle dysfunction. Thus, our data provide evidence of skeletal muscle abnormalities on advanced PH. AET was efficient in inducing an anti-cardiac remodeling effect besides preventing exercise intolerance. Significance: Our study provides a robust model of PH in mice, as well as highlights the importance of AET as a preventive strategy for exercise intolerance and, skeletal and cardiac muscle abnormalities in PH. (AU)

FAPESP's process: 15/22814-5 - Cancer and heart: new paradigms of diagnosis and treatment
Grantee:Carlos Eduardo Negrão
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/24284-6 - Role of aerobic physical training in muscle plasticity in experimental model of heart failure induced by pulmonary hypertension
Grantee:Janaina da Silva Vieira
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/25957-9 - Influence of aerobic fitness in redox homeostasis: identification and biological implications of cysteine residues oxidation in cardiac and skeletal muscle proteins
Grantee:Rodrigo Wagner Alves de Souza
Support type: Scholarships in Brazil - Post-Doctorate