BrazilianCopaiferaSpecies: Antifungal Activity against Clinically RelevantCandidaSpecies, Cellular Target, and In Vivo Toxicity

Author(s):Show less -

Andrade, Gessica ^[1] ; Orlando, Haniel Chadwick Silva ^[1] ; Scorzoni, Liliana ^{[2, 3]} ; Pedroso, Reginaldo Santos ^{[1, 4]} ; Abrao, Fariza ^[1] ; Carvalho, Marco Tulio Menezes ^[1] ; Veneziani, Rodrigo Cassio Sola ^[1] ; Ambrosio, Sergio Ricardo ^[1] ; Bastos, Jairo Kenupp ^[5] ; Mendes-Giannini, Maria Jose Soares ^[2] ; Martins, Carlos Henrique Gomes ^[6] ; Pires, Regina Helena ^[1]

Affiliation

^[1] Univ Franca, BR-14404600 Franca, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14801902 Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sci & Technol Inst Sao Jose dos Campos ICT, BR-12245000 Sao Jose Dos Campos - Brazil

^[4] Univ Fed Uberlandia, Hlth Tech Sch ESTES, BR-38400732 Uberlandia, MG - Brazil

^[5] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto - Brazil

^[6] Univ Fed Uberlandia, Inst Biomed Sci ICBIM, BR-38400902 Uberlandia, MG - Brazil

• Document type: Journal article
• Source: JOURNAL OF FUNGI; v. 6, n. 3 SEP 2020.
• Web of Science Citations: 0

Abstract

Plants belonging to the genusCopaiferaare widely used in Brazil due to their antimicrobial properties, among others. The re-emergence of classic fungal diseases as a consequence of antifungal resistance to available drugs has stimulated the search for plant-based compounds with antifungal activity, especially againstCandida. TheCandida-infectedCaenorhabditis elegansmodel was used to evaluate the in vitro antifungal potential ofCopaiferaleaf extracts and trunk oleoresins againstCandidaspecies. TheCopaiferaleaf extracts exhibited good antifungal activity against allCandidaspecies, with MIC values ranging from 5.86 to 93.75 mu g/mL. Both theCopaifera pauperaandCopaifera reticulataleaf extracts at 46.87 mu g/mL inhibitedCandida glabratabiofilm formation and showed no toxicity toC. elegans.The survival ofC. glabrata-infected nematodes increased at all the tested extract concentrations. Exposure toCopaiferaleaf extracts markedly increasedC. glabratacell vacuolization and cell membrane damage. Therefore,Copaiferaleaf extracts are potential candidates for the development of new and safe antifungal agents. (AU)

• FAPESP's process: 17/03250-9 - Potential of natural products derived from plants in the treatment of candidiasis
• Grantee: Haniel Chadwick Silva Orlando
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/02333-0 - Assessment in vitro and in vivo of the effects of vegetal products against free-living and biofilm growth mode of Candida
• Grantee: Regina Helena Pires
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants