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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Acute and chronic lithium treatment increases Wnt/beta-catenin transcripts in cortical and hippocampal tissue at therapeutic concentrations in mice

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Author(s):
De-Paula, Vanessa J. [1, 2, 3] ; dos Santos, Carla Cristine C. [4] ; Luque, Maria Carolina A. [5, 4] ; Ali, Taccyana M. [5, 4] ; Kalil, Jorge E. [2, 5, 6] ; Forlenza, Orestes V. [6] ; Cunha-Neto, Edecio [5, 6, 4]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept & Inst Psiquiatria, Hosp Clin HCFMUSP, Lab Psicobiol LIM23, Fac Med, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Dept & Inst Psiquiatria, Hosp Clin HCFMUSP, Lab Neurociencias LIM27, Fac Med, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Dept & Inst Psychiat, Lab Neurosci, Fac Med, Sao Paulo - Brazil
[4] Univ Sao Paulo, Div Imunol & Clin Alergia, Lab Imunol Clin & Alergia LIM60, Fac Med, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Fac Med, Inst Coracao InCor, Lab Imunol, Sao Paulo, SP - Brazil
[6] Inst Invest Imunol iii INCT, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: METABOLIC BRAIN DISEASE; v. 36, n. 1 NOV 2020.
Web of Science Citations: 0
Abstract

Lithium activates Wnt/beta-catenin signaling leading to stabilization of free cytosolic beta-catenin. The aim of the present study is to evaluate the in vivo effect of acute and chronic lithium treatment on the expression of beta-catenin target genes, addressing its transcripts HIG2, Bcl-xL, Cyclin D1, c-myc, in cortical and hippocampal tissue from adult mice. Lithium doses were established to yield therapeutic working concentrations. In acute treatment, mice received a 300 mu L of a 350 mg/kg solution of LiCl by gavage, and were euthanized after 2 h, 6 h and 12 h. To determine the effect of chronic treatment, animals were continuously fed either with chow supplemented with 2 g/kg Li2CO3, or regular chow (controls), being euthanized after 30 days. All animals had access to drinking water and 0.9% saline ad libitum. After acute and chronic treatments samples of peripheral blood were obtained from the tail vein for each animal, and serum concentrations of lithium were determined. All transcripts were up-regulated in cortical and hippocampal tissues of lithium-treated mice, both under acute and chronic treatments. There was a positive correlation between serum lithium concentrations and the increment in the expression of all transcripts. This effect was observed in all time points of the acute treatment (i.e., 2, 6 and 12 hours) and also after 30 days. We conclude that Wnt/beta-catenin transcriptional response (HIG2, Bcl-xL, Cyclin D1 and c-myc) is up-regulated in the mouse brain in response to acute and chronic lithium treatment at therapeutic concentrations. (AU)

FAPESP's process: 14/50890-5 - INCT of Investigation in Immunology
Grantee:Jorge Elias Kalil Filho
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/19892-3 - Effects of lithium on the expression and activity of the enzymes Phospholipase A2 and glycogen synthase kinase 3B and its relation to the phosphorylation state of Tau protein
Grantee:Vanessa de Jesus Rodrigues de Paula
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 09/52825-8 - Neurobiology of Alzheimer's disease: risk markers, prognosis and therapeutic response
Grantee:Wagner Farid Gattaz
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/01302-9 - Direct and indirect pathways of glycogen synthase kinase 3B inhibition by lithium in culture of neurons
Grantee:Vanessa de Jesus Rodrigues de Paula
Support type: Scholarships in Brazil - Post-Doctorate