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Role of interleukin-6 in the responses of the autophagic pathway in the heart of mice submitted in acute exhaustive physical exercise

Grant number: 19/17058-8
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2020
Effective date (End): February 28, 2022
Field of knowledge:Health Sciences - Physical Education
Principal researcher:Adelino Sanchez Ramos da Silva
Grantee:Rafael Lemes Rovina
Home Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Our research group verified that the imbalance between the excess of physical exercise and the adequate period of rest is linked with signals of pathological hypertrophy cardiac, lower phosphorylation of protein AMP-activated protein kinase (AMPK) in tyrosine 172, and higher concentrations of interleukin-6 (IL-6) in serum and cardiac tissue in mice. As known, which the increase in IL-6 is capable of inhibition the process of autophagy, whose activation is considered a promising therapeutic target for the prevention and treatment of cardiovascular diseases. In this sense, the main objective of this research project will be to investigate the role of IL-6 in the responses of the autophagic pathway in the heart of mice submitted in acute exhaustive physical exercise. For this, mice wild-type C57BL/6J (WT) and knockout IL-6 (KO) will be submitted to a protocol of acute exhaustive physical exercise. Serum samples and cardiac muscle will be collected immediately, 1 hour and 3 hours after the exhaustive exercise protocol. The functional parameters, serums and molecular will be analyzed by the echocardiogram, autophagic flux, further the techniques of Real-time-RT-PCR (Reverse Transcription Polymerase Chain Reaction) and immunoblotting. In addition, according to the statistical distribution of the data, parametric or non-parametric tests will be used to analyze the results. The significance level of p <0.05 will be adopted. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA ROCHA, ALISSON L.; ROVINA, RAFAEL L.; PINTO, ANA P.; MARAFON, BRUNO B.; DA SILVA, LILIAN E. C. M.; SIMABUCO, FERNANDO M.; FRANTZ, FABIANI G.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; FILHO, HUGO T.; DE FREITAS, ELLEN C.; RIVAS, DONATO A.; DA SILVA, ADELINO S. R. Interleukin-6 ablation does not alter morphofunctional heart characteristics but modulates physiological and inflammatory markers after strenuous exercise. CYTOKINE, v. 142, JUN 2021. Web of Science Citations: 0.

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