Grant number: | 17/09038-1 |
Support Opportunities: | Regular Research Grants |
Start date: | February 01, 2018 |
End date: | January 31, 2020 |
Field of knowledge: | Health Sciences - Physical Education |
Principal Investigator: | Adelino Sanchez Ramos da Silva |
Grantee: | Adelino Sanchez Ramos da Silva |
Host Institution: | Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
Associated researchers: | Giovana Rampazzo Teixeira ; José Rodrigo Pauli |
Associated research grant(s): | 19/00137-2 - Effects of physical exercise on the autophagy pathway, AP.R SPRINT |
Associated scholarship(s): | 19/10875-0 - Effects of regular physical exercise and overtraining on the behavior of the autophagic pathway in different tissues of mice,
BP.TT 18/03872-2 - Effects of regular physical exercise and overtraining on the behavior of the autophagic pathway in different tissues of mice, BP.TT |
Abstract
The imbalance between excessive training and inadequate recovery periods is linked to the performance decrement and to the states of functional overreaching (FOR), nonfunctional overreaching (NFOR) and/or overtraining syndrome (OTS). Mice submitted to an overtraining protocol (OT) based on chronic eccentric exercise sessions (OTR/down) showed hepatic activation of the mTORC1 pathway and increase of the AMPK (Thr172). In the heart tissue, both mTOR (Ser2448) and AMPK (Thr172) were inhibited. In the EDL, while the mTORC1 pathway was inhibited, the AMPK (Thr172) was not modulated. Knowing that the AMPK and mTOR pathway are respectively related to the activation and inhibition of the autophagic pathway, the main aim of this research project will be to compare the responses of the proteins related to the autophagic pathway in the liver, heart and EDL after the OTR/down protocol with other three physical exercise protocols, an aerobic (AER group) with predominant activation of the AMPK pathway, an resistance (FO group) with predominant activation of the mTOR pathway, and an concurrent (CR group). Mice will be divided into 8 experimental groups and the project will present two stages. The following methodologies will be used: immunoblotting, real-time polymerase chain reaction (qPCR), histology, immunohistochemistry, immunofluorescence, bioinformatics analysis and autophagic flow analysis. According to the statistical data distribution, parametric or nonparametric tests will be used to compare the investigated parameters between the experimental groups. The level of significance of pd0.05 will be adopted. (AU)
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