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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of interleukin-6 in inhibiting hepatic autophagy markers in exercised mice

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Author(s):
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Pinto, Ana P. [1] ; da Rocha, Alisson L. [1] ; Cabrera, Elisa M. B. [2] ; Marafon, Bruno B. [3] ; Kohama, Eike B. [1] ; Rovina, Rafael L. [3] ; Simabuco, Fernando M. [4] ; Bueno Junior, Carlos R. [3] ; de Moura, Leandro P. [4] ; Pauli, Jose R. [4] ; Cintra, Dennys E. [4] ; Ropelle, Eduardo R. [4] ; da Silva, Adelino S. R. [3, 1]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil
[2] Univ Guadalajara, Hlth Sci Univ Ctr, Inst Translat Nutrigenet & Nutrigen, Dept Mol Biol & Genom, Guadalajara, Jalisco - Mexico
[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Campinas UNICAMP, Lab Mol Biol Exercise LaBMEx, Sch Appl Sci, Limeira, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: CYTOKINE; v. 130, JUN 2020.
Web of Science Citations: 0
Abstract

Background: Based on the crosstalk of inflammation with apoptosis, autophagy, and endoplasmic reticulum (ER) stress, the main objective of this study was to explore the role of interleukin-6 (IL-6) on genes and proteins related to these phenomena in the livers of mice submitted to acute exhaustive exercise. Methods: Reverse transcription-quantitative polymerase chain reaction and immunoblotting technique were used to evaluate the livers of wild-type (WT) and IL-6 knockout (KO) mice at baseline (BL) and 3 h after the acute exhaustive physical exercise (EE). Results: Compared to the WT at baseline, the IL-6 KO had lower exhaustion velocity, mRNA levels of Mtor, Ulk1, Map1lc3b, and Mapk14, and protein contents of ATG5 and p-p70S6K/p70S6K. For the WT group, the EE decreased glycemia, mRNA levels of Casp3, Mtor, Ulk1, Foxo1a, Mapk14, and Ppargc1a, and protein contents of ATG5 and p-p70S6K/p70S6K, but increased mRNA levels of Sqstm1. For the IL-6 KO group, the EE decreased glycemia, mRNA levels of Casp3 and Foxo1a, and protein contents of pAkt/Akt and Mature/Pro IL-1beta, but increased mRNA levels of Sqstm1, and protein contents of p-AMPK/AMPK. Conclusion: The inhibition of the hepatic autophagy markers induced by the acute EE was attenuated in IL-6 KO mice, highlighting a new function of this cytokine. (AU)

FAPESP's process: 17/09038-1 - EFFECTS OF REGULAR PHYSICAL EXERCISE AND OVERTRAINING ON THE BEHAVIOR OF THE AUTOPHAGIC PATHWAY IN DIFFERENT TISSUES OF MICE.
Grantee:Adelino Sanchez Ramos da Silva
Support type: Regular Research Grants
FAPESP's process: 18/14818-9 - Study of molecular targets important for the control of cancer metabolism: the mTOR/S6K pathway as a central role
Grantee:Fernando Moreira Simabuco
Support type: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 18/12519-4 - Response of NR1D1 to the different acute physical exercise models in mice skeletal muscle
Grantee:Rafael Lemes Rovina
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/19869-8 - Molecular mechanisms related to increased hepatic fat content in response to excessive physical exercise
Grantee:Ana Paula Pinto
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/12765-2 - Emerging role of rev-erb-alpha in molecular adaptations to different physical exercise models
Grantee:Alisson Luiz da Rocha
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 19/10875-0 - Effects of regular physical exercise and overtraining on the behavior of the autophagic pathway in different tissues of mice
Grantee:Bruno Brieda Marafon
Support type: Scholarships in Brazil - Technical Training Program - Technical Training