Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of interleukin-6 in inhibiting hepatic autophagy markers in exercised mice

Full text
Show less -
Pinto, Ana P. [1] ; da Rocha, Alisson L. [1] ; Cabrera, Elisa M. B. [2] ; Marafon, Bruno B. [3] ; Kohama, Eike B. [1] ; Rovina, Rafael L. [3] ; Simabuco, Fernando M. [4] ; Bueno Junior, Carlos R. [3] ; de Moura, Leandro P. [4] ; Pauli, Jose R. [4] ; Cintra, Dennys E. [4] ; Ropelle, Eduardo R. [4] ; da Silva, Adelino S. R. [3, 1]
Total Authors: 13
[1] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil
[2] Univ Guadalajara, Hlth Sci Univ Ctr, Inst Translat Nutrigenet & Nutrigen, Dept Mol Biol & Genom, Guadalajara, Jalisco - Mexico
[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Campinas UNICAMP, Lab Mol Biol Exercise LaBMEx, Sch Appl Sci, Limeira, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: CYTOKINE; v. 130, JUN 2020.
Web of Science Citations: 0

Background: Based on the crosstalk of inflammation with apoptosis, autophagy, and endoplasmic reticulum (ER) stress, the main objective of this study was to explore the role of interleukin-6 (IL-6) on genes and proteins related to these phenomena in the livers of mice submitted to acute exhaustive exercise. Methods: Reverse transcription-quantitative polymerase chain reaction and immunoblotting technique were used to evaluate the livers of wild-type (WT) and IL-6 knockout (KO) mice at baseline (BL) and 3 h after the acute exhaustive physical exercise (EE). Results: Compared to the WT at baseline, the IL-6 KO had lower exhaustion velocity, mRNA levels of Mtor, Ulk1, Map1lc3b, and Mapk14, and protein contents of ATG5 and p-p70S6K/p70S6K. For the WT group, the EE decreased glycemia, mRNA levels of Casp3, Mtor, Ulk1, Foxo1a, Mapk14, and Ppargc1a, and protein contents of ATG5 and p-p70S6K/p70S6K, but increased mRNA levels of Sqstm1. For the IL-6 KO group, the EE decreased glycemia, mRNA levels of Casp3 and Foxo1a, and protein contents of pAkt/Akt and Mature/Pro IL-1beta, but increased mRNA levels of Sqstm1, and protein contents of p-AMPK/AMPK. Conclusion: The inhibition of the hepatic autophagy markers induced by the acute EE was attenuated in IL-6 KO mice, highlighting a new function of this cytokine. (AU)

Grantee:Adelino Sanchez Ramos da Silva
Support type: Regular Research Grants
FAPESP's process: 18/14818-9 - Study of molecular targets important for the control of cancer metabolism: the mTOR/S6K pathway as a central role
Grantee:Fernando Moreira Simabuco
Support type: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 18/12519-4 - Response of NR1D1 to the different acute physical exercise models in mice skeletal muscle
Grantee:Rafael Lemes Rovina
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/19869-8 - Molecular mechanisms related to increased hepatic fat content in response to excessive physical exercise
Grantee:Ana Paula Pinto
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/12765-2 - Emerging role of rev-erb-alpha in molecular adaptations to different physical exercise models
Grantee:Alisson Luiz da Rocha
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 19/10875-0 - Effects of regular physical exercise and overtraining on the behavior of the autophagic pathway in different tissues of mice
Grantee:Bruno Brieda Marafon
Support type: Scholarships in Brazil - Technical Training Program - Technical Training