| Grant number: | 17/12765-2 |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| Effective date (Start): | March 01, 2018 |
| Effective date (End): | February 28, 2022 |
| Field of knowledge: | Health Sciences - Physical Education |
| Principal Investigator: | Adelino Sanchez Ramos da Silva |
| Grantee: | Alisson Luiz da Rocha |
| Host Institution: | Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
Abstract Recently, the nuclear receptor rev-erb-alpha has been considered to play an important role in the skeletal muscle oxidative capacity adaptation, modulating the AMP-activated Protein Kinase (AMPK) signaling pathway. Classically, endurance physical exercise is able to activate this signaling pathway, but the effects of this and other physical exercise models (i.e., resistance and exhaustive) on rev-erb-alpha remain unknown. Besides the transcriptional regulation of this signaling pathway, rev-erb-alpha is also capable to regulate negatively the autophagy pathway in skeletal muscle and the dopamine biosynthesis in hippocampus. It is suggested in the literature that dopamine, together with serotonin, play a role in Central Nervous System (CNS) fatigue development. Briefly, rev-erb-alpha is candidate to modulate the molecular adaptations that regulate physical performance, CNS fatigue and autophagy. Thus, the main aim of the present project is to investigate the role of rev-erb-alpha in the molecular adaptations that regulate physical performance, CNS fatigue and autophagy after different exercise models. Mice will be divided into 5 groups: control (CT, sedentary); endurance I (END1, submitted to aerobic physical exercise protocol 1); endurance II (END2, submitted to aerobic physical exercise protocol 2); resistance (RES, submitted to strength physical exercise protocol) and exhaustive (OT; submitted to downhill overtraining protocol). As a translational characteristic, we will compare the messenger RNA of rev-erb-alpha and autophagic genes among sedentary and trained individuals. The following methodologies will be used: immunoblotting, real-time Polymerase Chain Reaction (qPCR), immunofluorescence and HPLC. According to the statistical data distribution, parametric or nonparametric tests will be used to compare the investigated parameters between the experimental groups. The level of significance of p < 0.05 will be adopted. (AU) | |
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