Tlr4 participates in the responses of markers of apoptosis, inflammation, and ER stress to different acute exercise intensities in mice hearts

Author(s):Show less -

de Vicente, Larissa G. ^[1] ; Pinto, Ana P. ^[1] ; Munoz, Vitor R. ^[2] ; Rovina, Rafael L. ^[3] ; da Rocha, Alisson L. ^[1] ; Gaspar, Rafael C. ^[2] ; da Silva, Lilian E. C. M. ^[4] ; Simabuco, Fernando M. ^[2] ; Frantz, Fabiani G. ^[5] ; Pauli, Jose R. ^[2] ; de Moura, Leandro P. ^[2] ; Cintra, Dennys E. ^[2] ; Ropelle, Eduardo R. ^[2] ; da Silva, Adelino S. R. ^{[3, 1]}

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, BR-14040900 Ribeirao Preto, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, BR-13484350 Limeira, SP - Brazil

^[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, BR-14040900 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Ophthalmol Otorhinolaryngol & Head & Neck Su, BR-14040900 Ribeirao Preto - Brazil

^[5] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Fac Pharmaceut Sci Ribeirao Preto, BR-14040900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 240, JAN 1 2020.
• Web of Science Citations: 0

Abstract

Background: Toll-like receptor 4 (Tlr4) is recognized due to its role in the immune response. Also, this protein can participate in the signaling pathway of events triggered by physical exercise such as apoptosis, inflammation, and endoplasmic reticulum (ER) stress. The main objective of this study was to evaluate the role of Tlr4 in the markers of these events in the myocardium of mice submitted to acute physical exercise (APE) protocols at different intensities. Methods: Echocardiogram, RT-qPCR, and immunoblotting technique were used to evaluate the left ventricle of wild-type (WT) and Tlr4 knockout (Tlr4 KO) submitted to APE protocols at 45, 60, and 75% of their maximal velocity. Also, we performed the bioinformatics analysis to establish the connection of heart mRNA levels of Tlr4 with heart genes of inflammation and ER stress of several isogenic strains of BXD mice. Results: Under basal conditions, the Tlr4 deletion diminished the performance, and expression of inflammation and ER stress genes in the left ventricle, but increased the serum levels of CK, Il-17, and Tnf-alpha. Under the same exercise conditions, the Tlr4 deletion reduced the glycemia, serum levels of CK, Il-17, and Tnf-alpha, as well as genes and/or proteins related to apoptosis, inflammation and ER stress in the left ventricle, but increased the levels of CK-mb and LDH, as well as other genes related to apoptosis, inflammation, and ER stress in the left ventricle. Conclusion: Altogether, the current findings highlighted the effects of different acute exercise intensities were attenuated in the heart of Tlr4 KO mice. (AU)

• FAPESP's process: 12/13558-7 - Molecular characterization of S6Ks in obesity and its associated diseases
• Grantee: Fernando Moreira Simabuco
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 17/09038-1 - EFFECTS OF REGULAR PHYSICAL EXERCISE AND OVERTRAINING ON THE BEHAVIOR OF THE AUTOPHAGIC PATHWAY IN DIFFERENT TISSUES OF MICE.
• Grantee: Adelino Sanchez Ramos da Silva
• Support type: Regular Research Grants
• FAPESP's process: 18/14818-9 - Study of molecular targets important for the control of cancer metabolism: the mTOR/S6K pathway as a central role
• Grantee: Fernando Moreira Simabuco
• Support type: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 18/12519-4 - Response of NR1D1 to the different acute physical exercise models in mice skeletal muscle
• Grantee: Rafael Lemes Rovina
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/19869-8 - Molecular mechanisms related to increased hepatic fat content in response to excessive physical exercise
• Grantee: Ana Paula Pinto
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/12765-2 - Emerging role of rev-erb-alpha in molecular adaptations to different physical exercise models
• Grantee: Alisson Luiz da Rocha
• Support type: Scholarships in Brazil - Doctorate