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| Author(s): |
Rafael Lemes Rovina
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP) |
| Defense date: | 2022-02-24 |
| Examining board members: |
Adelino Sanchez Ramos da Silva;
Jose Rodrigo Pauli;
Paulo Roberto Pereira Santiago
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| Advisor: | Adelino Sanchez Ramos da Silva |
| Abstract | |
The imbalance between excess physical exercise and the appropriate recovery period is associated with signs of pathological cardiac hypertrophy, decreased phosphorylation of Amp-activated protein kinase (AMPK) protein in tyrosine residue 172, and increased concentrations of interleukin-6 (IL6) in serum and cardiac tissue of mice. It is known that the increase in IL6 is capable of inhibiting the process of autophagy, whose activation is considered a promising therapeutic target for the prevention and treatment of cardiovascular diseases. In this sense, the main objective of this research project will be to investigate the role of IL6 in the responses of the autophagy pathway in the heart of mice submitted to exhaustive acute physical exercise. For this, wild-type mice C57BL/6J (WT) and IL6 knockout (KO) will undergo an exhaustive acute exercise protocol. Serum and heart muscle samples will be collected immediately, 1h and 3h after the exhaustive exercise protocol. The functional, seric and molecular parameters will be analyzed by echocardiogram, autophagic flow, in addition to the techniques of Real time-RTqPCR (Reverse Transcription Polymerase Chain Reaction) and immunoblotting. According to the statistical distribution of the data, parametric or nonparametric tests will be used for the analysis of the results. The significance level of p≤0, 05 will be adopted. In IL6 knockout mice, we observed lower exercise tolerance (p=0.003), higher oxygen consumption at baseline (p=0.001), and three hours after the exercise session Mtco1, Mtnd1 and Nampt mRNA levels were lower (p=0.001; p=0.002; p=0.005). Therefore, IL6 appears to play an important role in physical performance and oxygen consumption, but no change in autophagic flux was observed in response to exercise. (AU) | |
| FAPESP's process: | 19/17058-8 - Role of interleukin-6 in the responses of the autophagic pathway in the heart of mice submitted in acute exhaustive physical exercise |
| Grantee: | Rafael Lemes Rovina |
| Support Opportunities: | Scholarships in Brazil - Master |