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Effects of overtraining protocol based on eccentric contractions on the inflammatory and Mtor pathways in the heart of C57BL/6 mice

Grant number: 15/13275-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2015
Effective date (End): September 30, 2016
Field of knowledge:Health Sciences - Physical Education
Principal researcher:Adelino Sanchez Ramos da Silva
Grantee:Gustavo Paroschi Morais
Home Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The eccentric exercise-induced nonfunctional overreaching in mice is associated with low-grade chronic inflammation in skeletal muscle samples and serum. In addition, it is known that myocardium is also able to produce proinflammatory cytokines and that mTOR (mammalian target of rapamycin) pathway has a cardioprotector effect against these cytokines. However, the myocardium adaptations in response to EE-induced NFOR remain unknown. Thus, the main aim of the present investigation was to verify the effects of the overtraining (OT) protocol based on chronic sessions of EE on the content and activation of the proteins related to cardiac hypertrophy in mice - Akt (Protein kinase B) mTOR (mammalian target of rapamycin), S6K (S6 kinase), 4EBP1 (4E binding protein) - and on the content of the proinflammatory cytokines in mice cardiac muscle. The animals will be divided into 4 groups: naive (N, sedentary mice), control (CT, sedentary mice subjected to physical tests), trained (TR, mice subjected to the training protocol) and overtraining (OT, mice subjected to the OT protocol based on downhill running). The mice will be evaluated daily for variation in body weight and food intake. The content and activation of aforementioned proteins will be evaluated in the left ventricle of the heart muscle by the immunoblotting technique. For comparison between groups will be used variance analysis followed by test for multiple comparisons of means. A significance level of 5% will be used.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA ROCHA, ALISSON L.; PINTO, ANA P.; TEIXEIRA, GIOVANA R.; PEREIRA, BRUNO C.; OLIVEIRA, LUCIANA C.; SILVA, ADRIANA C.; MORAIS, GUSTAVO P.; CINTRA, DENNYS E.; PAULI, JOSE R.; DA SILVA, ADELINO S. R. Exhaustive Training Leads to Hepatic Fat Accumulation. Journal of Cellular Physiology, v. 232, n. 8, p. 2094-2103, AUG 2017. Web of Science Citations: 8.
PINTO, ANA PAULA; DA ROCHA, ALISSON LUIZ; PEREIRA, BRUNO CESAR; OLIVEIRA, LUCIANA DA COSTA; MORAIS, GUSTAVO PAROSCHI; MOURA, LEANDRO PEREIRA; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO; RAMOS DA SILVA, ADELINO SANCHEZ. Excessive training is associated with endoplasmic reticulum stress but not apoptosis in the hypothalamus of mice. APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, v. 42, n. 4, p. 354-360, APR 2017. Web of Science Citations: 5.

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