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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Excessive training is associated with endoplasmic reticulum stress but not apoptosis in the hypothalamus of mice

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Author(s):
Pinto, Ana Paula ; da Rocha, Alisson Luiz ; Pereira, Bruno Cesar ; Oliveira, Luciana da Costa ; Morais, Gustavo Paroschi ; Moura, Leandro Pereira ; Ropelle, Eduardo Rochete ; Pauli, Jose Rodrigo ; Ramos da Silva, Adelino Sanchez
Total Authors: 9
Document type: Journal article
Source: APPLIED PHYSIOLOGY NUTRITION AND METABOLISM; v. 42, n. 4, p. 354-360, APR 2017.
Web of Science Citations: 5
Abstract

Downhill running-based overtraining model increases the hypothalamic levels of IL-1 beta, TNF-alpha, SOCS3, and pSAPK-JNK. The aim of the present study was to verify the effects of 3 overtraining protocols on the levels of BiP, pIRE-1 (Ser724), pPERK (Thr981), pelF2 alpha (Ser52), ATF-6, GRP-94, caspase 4, caspase 12, pAKT (Ser473), pmTOR (Ser2448), and pAMPK (Thr172) proteins in the mouse hypothalamus. The mice were randomized into the control, overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up), and overtrained by running without inclination (OTR) groups. After the overtraining protocols (i.e., at the end of week 8), hypothalamus was removed and used for immunoblotting. The OTR/down group exhibited increased levels of all of the analyzed endoplasmic reticulum stress markers in the hypothalamus at the end of week 8. The OTR/up and OTR groups exhibited increased levels of BiP, pIRE-1 (Ser724), and pPERK (Thr981) in the hypothalamus at the end of week 8. There were no significant differences in the levels of caspase 4, caspase 12, pAKT (Ser473), pmTOR (Ser2448), and pAMPK (Thr172) between the experimental groups at the end of week 8. In conclusion, the 3 overtraining protocols increased the endoplasmic reticulum stress at the end of week 8. (AU)

FAPESP's process: 13/19985-7 - Effects of overtraining induced by exercise in treadmill without inclination, uphill and downhill on the content and activation of proteins of insulin signaling pathway and inflamation in mice
Grantee:Bruno Cesar Pereira
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/08013-0 - Overreaching not functional in animal model and endoplasmic reticulum stress in muscle heart and liver
Grantee:Ana Paula Pinto
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/25459-9 - Nonfunctional overreaching in animal model: inflammatory and hypertrophic adaptions of the cardiac muscle
Grantee:Alisson Luiz da Rocha
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/13275-3 - Effects of overtraining protocol based on eccentric contractions on the inflammatory and Mtor pathways in the heart of C57BL/6 mice
Grantee:Gustavo Paroschi Morais
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/20591-3 - Responses of proteins from the inflammatory, insulinic and hypertrophic molecular pathways to nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in skeletal muscle of mice
Grantee:Adelino Sanchez Ramos da Silva
Support type: Regular Research Grants