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Responses of proteins from the inflammatory, insulinic and hypertrophic molecular pathways to nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in skeletal muscle of mice

Abstract

Nonfunctional overreaching (NFOR) induced by downhill running is associated with low-grade chronic inflammation and insulin signalling pathway impairment in skeletal muscles of mice. However, up to today, it is not possible to state that NFOR not induced by eccentric exercise (EE) is associated with these molecular alterations. Thus, the main aim of the present investigation is to compare the effects of NFOR induced by EE with other two protocols with similar intensity and volume, but performed in uphill and without inclination on the cytokine serum levels and on the proteins from the inflammatory, insulinic and hypertrophic molecular pathways. The animals will be divided into four groups: Control (C; n=15; sedentary), Overtraining in downhill (OT1; n=15; overtrained in downhill), Overtraining without inclination (OT2; n=15; overtrained without inclination), Overtraining in uphill (OT3; n=15; overtrained in uphill). The mice will be evaluated daily for variation in body weight and food intake. The performance of rodents will be measured in the beginning (week 0), at the middle (week 4) and at the end (week 8) of the experimental protocols by the following evaluations: incremental load test, rotarod test, exhaustive test and grip force. At the end of week 8, mice will be submitted to the insulin tolerance test. The content and activation of the proteins from the mentioned metabolic pathways will be determined in the extensor digitorum longus and soleus samples by the immunoblotting technique. For comparison between groups will be used variance analysis followed by test for multiple comparisons of means. A significance level of 5% will be used. (AU)

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Scientific publications (15)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA ROCHA, ALISSON L.; PINTO, ANA P.; KOHAMA, EIKE B.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R. The proinflammatory effects of chronic excessive exercise. CYTOKINE, v. 119, p. 57-61, JUL 2019. Web of Science Citations: 4.
OLIVEIRA, LUCIANA DA C.; DE MORAIS, GUSTAVO P.; DA ROCHA, ALISSON L.; TEIXEIRA, GIOVANA R.; PINTO, ANA P.; DE VICENTE, LARISSA G.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; MEKARY, RANIA A.; ROPELLE, EDUARDO R.; CINTRA, DENNYS E.; DA SILVA, ADELINO S. R. Excessive treadmill training enhances the insulin signaling pathway and glycogen deposition in mice hearts. Journal of Cellular Biochemistry, v. 120, n. 2, p. 1304-1317, FEB 2019. Web of Science Citations: 1.
DA ROCHA, ALISSON L.; TEIXEIRA, GIOVANA R.; PINTO, ANA P.; DE MORAIS, GUSTAVO P.; OLIVEIRA, LUCIANA DA C.; DE VICENTE, LARISSA GAIOTO; DA SILVA, LILIAN E. C. M.; PAULI, JOSE R.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DE MOURA, LEANDRO P.; MEKARY, RANIA A.; DE FREITAS, ELLEN C.; DA SILVA, ADELINO S. R. Excessive training induces molecular signs of pathologic cardiac hypertrophy. Journal of Cellular Physiology, v. 233, n. 11, p. 8850-8861, NOV 2018. Web of Science Citations: 5.
DA ROCHA, ALISSON L.; PINTO, ANA P.; PEREIRA, BRUNO C.; KOHAMA, EIKE B.; MORAIS, GUSTAVO P.; DE VICENTE, LARISSA G.; PAULI, JOSE R.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DE MOURA, LEANDRO P.; FILHO, HUGO T.; DA SILVA, ADELINO S. R. Positive effects of total recovery period on anti- and pro-inflammatory cytokines are not linked to performance re-establishment in overtrained mice. CYTOKINE, v. 103, p. 69-76, MAR 2018. Web of Science Citations: 1.
MORAIS, G. P.; DA ROCHA, A.; PINTO, A. P.; OLIVEIRA, L. DA C.; DE VICENTE, L. G.; FERREIRA, G. N.; DE FREITAS, E. C.; DA SILVA, A. S. R. Uphill Running Excessive Training Increases Gastrocnemius Glycogen Content in C57BL/6 Mice. Physiological Research, v. 67, n. 1, p. 107-115, 2018. Web of Science Citations: 0.
DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; TEIXEIRA, GIOVANA R.; PINTO, ANA P.; FRANTZ, FABIANI G.; ELIAS, LUCILA L. K.; LIRA, FABIO S.; PAULI, JOSE R.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DE MOURA, LEANDRO P.; MEKARY, RANIA A.; DE FREITAS, ELLEN C.; DA SILVA, ADELINO S. R. Treadmill slope Modulates inflammation, Fiber Type composition, androgen, and glucocorticoid receptors in the skeletal Muscle of Overtrained Mice. FRONTIERS IN IMMUNOLOGY, v. 8, OCT 25 2017. Web of Science Citations: 7.
PINTO, ANA P.; DA ROCHA, ALISSON L.; OLIVEIRA, LUCIANA DA C.; MORAIS, GUSTAVO P.; DE VICENTE, LARISSA G.; CINTRA, DENNYS E.; PAULI, JOSE R.; MOURA, LEANDRO P.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R. Levels of Hepatic Activating Transcription Factor 6 and Caspase-3 Are Downregulated in Mice after Excessive Training. FRONTIERS IN ENDOCRINOLOGY, v. 8, SEP 26 2017. Web of Science Citations: 2.
DA ROCHA, ALISSON L.; PINTO, ANA P.; TEIXEIRA, GIOVANA R.; PEREIRA, BRUNO C.; OLIVEIRA, LUCIANA C.; SILVA, ADRIANA C.; MORAIS, GUSTAVO P.; CINTRA, DENNYS E.; PAULI, JOSE R.; DA SILVA, ADELINO S. R. Exhaustive Training Leads to Hepatic Fat Accumulation. Journal of Cellular Physiology, v. 232, n. 8, p. 2094-2103, AUG 2017. Web of Science Citations: 8.
PINTO, ANA PAULA; DA ROCHA, ALISSON LUIZ; PEREIRA, BRUNO CESAR; OLIVEIRA, LUCIANA DA COSTA; MORAIS, GUSTAVO PAROSCHI; MOURA, LEANDRO PEREIRA; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO; RAMOS DA SILVA, ADELINO SANCHEZ. Excessive training is associated with endoplasmic reticulum stress but not apoptosis in the hypothalamus of mice. APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, v. 42, n. 4, p. 354-360, APR 2017. Web of Science Citations: 5.
PEREIRA, BRUNO C.; DA ROCHA, ALISSON L.; PINTO, ANA P.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; MEKARY, RANIA A.; DE FREITAS, ELLEN C.; DA SILVA, ADELINO S. R. Excessive training impairs the insulin signal transduction in mice skeletal muscles. Journal of Endocrinology, v. 230, n. 1, p. 93-104, JUL 2016. Web of Science Citations: 8.
DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; PAULI, JOSE R.; DE SOUZA, CLAUDIO T.; TEIXEIRA, GIOVANA R.; LIRA, FABIO S.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; CARLOS, JR., R. B.; DA SILVA, ADELINO S. R. Downhill Running Excessive Training Inhibits Hypertrophy in Mice Skeletal Muscles with Different Fiber Type Composition. Journal of Cellular Physiology, v. 231, n. 5, p. 1045-1056, MAY 2016. Web of Science Citations: 14.
PEREIRA, BRUNO C.; DA ROCHA, ALISSON L.; PINTO, ANA P.; PAULI, JOSE R.; DE SOUZA, CLAUDIO T.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DE FREITAS, ELLEN C.; ZAGATTO, ALESSANDRO M.; DA SILVA, ADELINO S. R. Excessive eccentric exercise-induced overtraining model leads to endoplasmic reticulum stress in mice skeletal muscles. Life Sciences, v. 145, p. 144-151, JAN 15 2016. Web of Science Citations: 14.
PEREIRA, B. C.; DA ROCHA, A. L.; PAULI, J. R.; ROPELLE, E. R.; DE SOUZA, C. T.; CINTRA, D. E.; SANT'ANA, M. R.; DA SILVA, A. S. R. EXCESSIVE ECCENTRIC EXERCISE LEADS TO TRANSITORY HYPOTHALAMIC INFLAMMATION, WHICH MAY CONTRIBUTE TO THE LOW BODY WEIGHT GAIN AND FOOD INTAKE IN OVERTRAINED MICE. Neuroscience, v. 311, p. 231-242, DEC 17 2015. Web of Science Citations: 17.
DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; PAULI, JOSE R.; CINTRA, DENNYS E.; DE SOUZA, CLAUDIO T.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R. Downhill Running-Based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway without Concomitant Decrease of Inflammatory Proteins. PLoS One, v. 10, n. 10 OCT 7 2015. Web of Science Citations: 9.
PEREIRA, B. C.; LUCAS, G.; DA ROCHA, A. L.; PAULI, J. R.; ROPELLE, E. R.; CINTRA, D.; DE SOUZA, C. T.; BUENO, C. R.; DA SILVA, A. S. Eccentric Exercise Leads to Glial Activation but not Apoptosis in Mice Spinal Cords. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. 36, n. 5, p. 378-385, MAY 2015. Web of Science Citations: 17.

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