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Responses of proteins from the inflammatory, insulinic and hypertrophic molecular pathways to nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in skeletal muscle of mice

Abstract

Nonfunctional overreaching (NFOR) induced by downhill running is associated with low-grade chronic inflammation and insulin signalling pathway impairment in skeletal muscles of mice. However, up to today, it is not possible to state that NFOR not induced by eccentric exercise (EE) is associated with these molecular alterations. Thus, the main aim of the present investigation is to compare the effects of NFOR induced by EE with other two protocols with similar intensity and volume, but performed in uphill and without inclination on the cytokine serum levels and on the proteins from the inflammatory, insulinic and hypertrophic molecular pathways. The animals will be divided into four groups: Control (C; n=15; sedentary), Overtraining in downhill (OT1; n=15; overtrained in downhill), Overtraining without inclination (OT2; n=15; overtrained without inclination), Overtraining in uphill (OT3; n=15; overtrained in uphill). The mice will be evaluated daily for variation in body weight and food intake. The performance of rodents will be measured in the beginning (week 0), at the middle (week 4) and at the end (week 8) of the experimental protocols by the following evaluations: incremental load test, rotarod test, exhaustive test and grip force. At the end of week 8, mice will be submitted to the insulin tolerance test. The content and activation of the proteins from the mentioned metabolic pathways will be determined in the extensor digitorum longus and soleus samples by the immunoblotting technique. For comparison between groups will be used variance analysis followed by test for multiple comparisons of means. A significance level of 5% will be used. (AU)

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Scientific publications (15)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; PAULI, JOSE R.; DE SOUZA, CLAUDIO T.; TEIXEIRA, GIOVANA R.; LIRA, FABIO S.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; CARLOS, JR., R. B.; DA SILVA, ADELINO S. R.. Downhill Running Excessive Training Inhibits Hypertrophy in Mice Skeletal Muscles with Different Fiber Type Composition. Journal of Cellular Physiology, v. 231, n. 5, p. 1045-1056, . (13/20591-3, 13/19985-7, 14/25459-9, 13/22737-5)
PEREIRA, B. C.; DA ROCHA, A. L.; PAULI, J. R.; ROPELLE, E. R.; DE SOUZA, C. T.; CINTRA, D. E.; SANT'ANA, M. R.; DA SILVA, A. S. R.. EXCESSIVE ECCENTRIC EXERCISE LEADS TO TRANSITORY HYPOTHALAMIC INFLAMMATION, WHICH MAY CONTRIBUTE TO THE LOW BODY WEIGHT GAIN AND FOOD INTAKE IN OVERTRAINED MICE. Neuroscience, v. 311, p. 231-242, . (13/20591-3, 13/19985-7, 14/25459-9)
DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; PAULI, JOSE R.; CINTRA, DENNYS E.; DE SOUZA, CLAUDIO T.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R.. Downhill Running-Based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway without Concomitant Decrease of Inflammatory Proteins. PLoS One, v. 10, n. 10, . (13/20591-3, 13/19985-7, 14/25459-9, 13/22737-5)
DA ROCHA, ALISSON L.; PINTO, ANA P.; PEREIRA, BRUNO C.; KOHAMA, EIKE B.; MORAIS, GUSTAVO P.; DE VICENTE, LARISSA G.; PAULI, JOSE R.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DE MOURA, LEANDRO P.; et al. Positive effects of total recovery period on anti- and pro-inflammatory cytokines are not linked to performance re-establishment in overtrained mice. CYTOKINE, v. 103, p. 69-76, . (13/20591-3, 13/19985-7)
PEREIRA, BRUNO C.; DA ROCHA, ALISSON L.; PINTO, ANA P.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; MEKARY, RANIA A.; DE FREITAS, ELLEN C.; DA SILVA, ADELINO S. R.. Excessive training impairs the insulin signal transduction in mice skeletal muscles. Journal of Endocrinology, v. 230, n. 1, p. 93-104, . (13/20591-3, 13/19985-7, 14/25459-9, 15/08013-0)
DA ROCHA, ALISSON L.; PINTO, ANA P.; TEIXEIRA, GIOVANA R.; PEREIRA, BRUNO C.; OLIVEIRA, LUCIANA C.; SILVA, ADRIANA C.; MORAIS, GUSTAVO P.; CINTRA, DENNYS E.; PAULI, JOSE R.; DA SILVA, ADELINO S. R.. Exhaustive Training Leads to Hepatic Fat Accumulation. Journal of Cellular Physiology, v. 232, n. 8, p. 2094-2103, . (13/19985-7, 14/25459-9, 15/13275-3, 15/08013-0, 13/20591-3)
DA ROCHA, ALISSON L.; TEIXEIRA, GIOVANA R.; PINTO, ANA P.; DE MORAIS, GUSTAVO P.; OLIVEIRA, LUCIANA DA C.; DE VICENTE, LARISSA GAIOTO; DA SILVA, LILIAN E. C. M.; PAULI, JOSE R.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; et al. Excessive training induces molecular signs of pathologic cardiac hypertrophy. Journal of Cellular Physiology, v. 233, n. 11, p. 8850-8861, . (13/20591-3, 14/25459-9)
OLIVEIRA, LUCIANA DA C.; DE MORAIS, GUSTAVO P.; DA ROCHA, ALISSON L.; TEIXEIRA, GIOVANA R.; PINTO, ANA P.; DE VICENTE, LARISSA G.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; MEKARY, RANIA A.; ROPELLE, EDUARDO R.; et al. Excessive treadmill training enhances the insulin signaling pathway and glycogen deposition in mice hearts. Journal of Cellular Biochemistry, v. 120, n. 2, p. 1304-1317, . (13/20591-3)
PINTO, ANA P.; DA ROCHA, ALISSON L.; OLIVEIRA, LUCIANA DA C.; MORAIS, GUSTAVO P.; DE VICENTE, LARISSA G.; CINTRA, DENNYS E.; PAULI, JOSE R.; MOURA, LEANDRO P.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R.. Levels of Hepatic Activating Transcription Factor 6 and Caspase-3 Are Downregulated in Mice after Excessive Training. FRONTIERS IN ENDOCRINOLOGY, v. 8, . (13/20591-3, 15/08013-0)
DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; TEIXEIRA, GIOVANA R.; PINTO, ANA P.; FRANTZ, FABIANI G.; ELIAS, LUCILA L. K.; LIRA, FABIO S.; PAULI, JOSE R.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; et al. Treadmill slope Modulates inflammation, Fiber Type composition, androgen, and glucocorticoid receptors in the skeletal Muscle of Overtrained Mice. FRONTIERS IN IMMUNOLOGY, v. 8, . (13/20591-3, 13/19985-7)
PINTO, ANA PAULA; DA ROCHA, ALISSON LUIZ; PEREIRA, BRUNO CESAR; OLIVEIRA, LUCIANA DA COSTA; MORAIS, GUSTAVO PAROSCHI; MOURA, LEANDRO PEREIRA; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO; RAMOS DA SILVA, ADELINO SANCHEZ. Excessive training is associated with endoplasmic reticulum stress but not apoptosis in the hypothalamus of mice. APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, v. 42, n. 4, p. 354-360, . (13/20591-3, 15/13275-3, 13/19985-7, 15/08013-0, 14/25459-9)
DA ROCHA, ALISSON L.; PINTO, ANA P.; KOHAMA, EIKE B.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R.. The proinflammatory effects of chronic excessive exercise. CYTOKINE, v. 119, p. 57-61, . (12/23258-0, 13/19985-7, 17/12765-2, 14/25459-9, 10/08239-4, 13/22737-5, 15/08013-0, 13/20591-3, 17/19869-8, 11/02652-0, 17/09038-1)
MORAIS, G. P.; DA ROCHA, A.; PINTO, A. P.; OLIVEIRA, L. DA C.; DE VICENTE, L. G.; FERREIRA, G. N.; DE FREITAS, E. C.; DA SILVA, A. S. R.. Uphill Running Excessive Training Increases Gastrocnemius Glycogen Content in C57BL/6 Mice. Physiological Research, v. 67, n. 1, p. 107-115, . (14/25459-9, 13/20591-3, 15/08013-0)
PEREIRA, BRUNO C.; DA ROCHA, ALISSON L.; PINTO, ANA P.; PAULI, JOSE R.; DE SOUZA, CLAUDIO T.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DE FREITAS, ELLEN C.; ZAGATTO, ALESSANDRO M.; DA SILVA, ADELINO S. R.. Excessive eccentric exercise-induced overtraining model leads to endoplasmic reticulum stress in mice skeletal muscles. Life Sciences, v. 145, p. 144-151, . (13/20591-3, 13/19985-7, 14/25459-9, 15/08013-0)
PEREIRA, B. C.; LUCAS, G.; DA ROCHA, A. L.; PAULI, J. R.; ROPELLE, E. R.; CINTRA, D.; DE SOUZA, C. T.; BUENO, C. R.; DA SILVA, A. S.. Eccentric Exercise Leads to Glial Activation but not Apoptosis in Mice Spinal Cords. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. 36, n. 5, p. 378-385, . (13/20591-3, 13/19985-7, 13/22737-5)

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