Effects of nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in content and activation of the mTOR pathway proteins in skeletal muscle of mice

• Grant number: 13/22737-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): April 01, 2014
• Effective date (End): December 31, 2014
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Adelino Sanchez Ramos da Silva
• Grantee: Alisson Luiz da Rocha
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Recently, Pereira et al. (2013) verified that nonfunctional overreaching (NFOR) is associate with performance decrement and upregulation of myostatin. However, up to today, it is not possible to state that NFOR not induced by eccentric exercise is associated with the dysfunctions cited previously. Moreover, Winbanks et al. (2012) concluded that skeletal muscle growth mediates by follistatin is regulated by mTOR (mammalian target of rapamycin) and Smad 3 independently of myostatin overexpression or inhibition. Thus, the aim is to compare the effects of the overtraining protocol proposed by Pereira et al. (2012) performed in downhill with other two protocols with similar intensity and volume, but performed in uphill and without inclination, on the protein content and activation of proteins related on inhibition or skeletal muscle growth - 4EBP1 (4E binding protein), myostatin, mTOR, S6k (ribosomal S6 kinase), S6RP (S6 ribosomal protein), Smad 3, e TSC2 (tuberin). The animals will be divided into four groups: Control (C; sedentary), Overtraining in downhill (OT1; overtrained in downhill), Overtraining without inclination (OT2; overtrained without inclination), Overtraining in uphill (OT3; overtrained in uphill). The mice will be evaluated daily for variation in body weight and food intake. The protein content will be measured in solemn and extensor digitorum longus (EDL) muscles by immunoblotting technique. For comparison between groups will be used variance analysis followed by a test for multiple comparisons of means. A significance level of 5% will be used. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; PAULI, JOSE R.; DE SOUZA, CLAUDIO T.; TEIXEIRA, GIOVANA R.; LIRA, FABIO S.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; CARLOS, JR., R. B.; DA SILVA, ADELINO S. R.. Downhill Running Excessive Training Inhibits Hypertrophy in Mice Skeletal Muscles with Different Fiber Type Composition. Journal of Cellular Physiology, v. 231, n. 5, p. 1045-1056, MAY 2016. (13/20591-3, 13/19985-7, 14/25459-9, 13/22737-5)

DA ROCHA, ALISSON L.; PEREIRA, BRUNO C.; PAULI, JOSE R.; CINTRA, DENNYS E.; DE SOUZA, CLAUDIO T.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R.. Downhill Running-Based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway without Concomitant Decrease of Inflammatory Proteins. PLoS One, v. 10, n. 10, OCT 7 2015. (13/20591-3, 13/19985-7, 14/25459-9, 13/22737-5)

PEREIRA, B. C.; LUCAS, G.; DA ROCHA, A. L.; PAULI, J. R.; ROPELLE, E. R.; CINTRA, D.; DE SOUZA, C. T.; BUENO, C. R.; DA SILVA, A. S.. Eccentric Exercise Leads to Glial Activation but not Apoptosis in Mice Spinal Cords. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. 36, n. 5, p. 378-385, MAY 2015. (13/20591-3, 13/19985-7, 13/22737-5)

DA ROCHA, ALISSON L.; PINTO, ANA P.; KOHAMA, EIKE B.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R.. The proinflammatory effects of chronic excessive exercise. CYTOKINE, v. 119, p. 57-61, JUL 2019. (12/23258-0, 13/19985-7, 17/12765-2, 14/25459-9, 10/08239-4, 13/22737-5, 15/08013-0, 13/20591-3, 17/19869-8, 11/02652-0, 17/09038-1)