Voriconazole-natural latex dressings for treating infected Candida spp. skin ulcers

Author(s)

da Silva, V, Thaina ; de Barros, Natan R. ^{[1, 2]} ; Costa-Orlandi, Caroline B. ^[1] ; Tanaka, Jean L. ^[1] ; Moro, Lincoln G. ^[1] ; Pegorin, Giovana S. ^{[1, 2]} ; Oliveira, Kassandra S. M. ^[3] ; Mendes-Gianinni, Maria J. S. ^[1] ; Fusco-Almeida, Aria M. ^[1] ; Herculano, Rondinelli D. ^[1]

Affiliation

^[1] da Silva, Thaina, V, Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Biotechnol & Bioproc Engn Dept, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Chem, Biochem & Chem Technol Dept, BR-14800060 Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Agr, Rural Engn & Socioecon Dept, BR-18610034 Botucatu, SP - Brazil

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 15, n. 15, p. 1439-1452, OCT 2020.
• Web of Science Citations: 0

Abstract

Aim: This work aimed to develop a membrane based on voriconazole (VCZ)-loaded natural rubber latex (NRL) for treating infected ulcers with Candida spp. and study their interaction, drug release, antifungal activity against Candida parapsilosis and biological characterization. Materials \& methods: VCZ-loaded NRL membrane was produced by casting method. Results: Infrared spectrum showed that the incorporation of VCZ into the NRL membrane maintained its characteristics. Its mechanical properties were considered suitable for dermal application. The VCZ was able to release from NRL membrane, maintaining its antifungal activity against C. parapsilosis, besides did not present hemolytic effects. Conclusion: The VCZ-NRL membrane showed good results in mechanical, antifungal and biological assays, representing an interesting alternative to treatment of infected wound with Candida spp. Lay abstract Aim: The aim of this work was to develop a membrane from natural rubber latex (NRL) containing an antifungal for the treatment of infected ulcers with Candida spp. and study their interaction, drug release, antifungal activity against Candida parapsilosis and effects on red blood cells. Materials \& methods: The membrane was produced by mixing voriconazole (VCZ) and NRL on a plate. Results: It was shown that the incorporation of VCZ maintained its physical-chemical characteristics, which were considered suitable for dermal application. The VCZ was released from membrane, preserving antifungal activity against C. parapsilosis, besides did not damage the red blood cells membrane. Conclusion: The VCZ-NRL membrane showed good results being an alternative to treatment of infected wound with Candida spp. (AU)

• FAPESP's process: 17/19603-8 - Development of a novel patch containing latex and barbatimam e fractions and study of signaling pathways in cutaneous wound repair
• Grantee: Rondinelli Donizetti Herculano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/18388-6 - Role of biofilm in the pathogenesis of dermatophytosis and development of combat strategies
• Grantee: Caroline Barcelos Costa Orlandi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral