Ultrastructure and histochemistry of the male reproductive system of the genusCallinectesStimpson, 1860 (Brachyura: Portunidae)

Author(s)

Watanabe, Timoteo T. ^{[1, 2]} ; Nascimento, Fabiana A. ^[1] ; Mantelatto, Fernando L. ^[3] ; Zara, Fernando J. ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Agr & Vet Sch, Jaboticabal & Aquaculture Ctr CAUNESP, Invertebrate Morphol Lab IML, Dept Appl Biol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14881900 Jaboticabal, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Biosci Inst Rio Claro, Postgrad Course Biol Sci Zool, Rio Claro, SP - Brazil

^[3] Univ Sao Paulo, Sci & Letters Ribeirao Preto FFCLRP, Fac Philosophy, Lab Bioecol & Crustacean Systemat LBSC, Dept Biol, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Journal of Morphology; v. 281, n. 12, p. 1660-1678, DEC 2020.
• Web of Science Citations: 0

Abstract

We described the ultrastructure and histochemistry of the reproductive system of fiveCallinectesspecies, and evaluate the seasonal variation in weight of the reproductive system and hepatopancreas by comparing annual changes of somatic indices. The somatic indices changed little throughout the year. InCallinectes, spermatogenesis occurs inside the lobular testes and, within each lobule, the cells are at the same developmental stage. Spermatogenesis and spermiogenesis follow the same development pattern in allCallinectesstudied. Mature spermatozoa are released into the seminiferous ducts through the collecting ducts. Cells of the vas deferens are secretory as evidenced by rough endoplasmic reticulum, Golgi complex, and secretory vesicles that produce the seminal fluid. The anterior vas deferens shows two portions: proximal and distal. In proximal portion (AVDp), spermatozoa are clustered and embedded in an electron-dense, basophilic glycoproteinaceous secretion Type I. In the distal portion (AVDd), the spermatophore wall is formed by incorporation of a less electron-dense glycoproteinaceous secretion Type II. The secretion Type I change to an acid polysaccharide-rich matrix that separates the spermatophores from each other. The median vas deferens (MVD) stores the spermatophores and produces the granular glycoproteinaceous seminal fluid. The posterior vas deferens (PVD) has few spermatophores. Its epithelium has many mitochondria and the PVD seminal fluid changes into a liquid and homogeneous glycoprotein. Many outpocketings in the PVD and MVD help to increase the fluid production. Overall, the reproductive pattern ofCallinectesis similar to other species that produce sperm plugs. The secretions of AVD, MVD, and PVD are responsible for the polymerization that forms the solid, waxy plug in the seminal receptacle. The traits identified here are common to all Portunidae species studied so far. (AU)

• FAPESP's process: 09/01348-5 - Male reproductive system of Callinectes ornatus Ordway, 1863 (Crustacea - Decapoda): ultrastructure and histochemistry
• Grantee: Fabiana Aparecida Do Nascimento
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 10/50188-8 - Decapod crustaceans: multi-disciplinary characterization of the sea biodiversity of the State of São Paulo (taxonomy, spermiotaxonomy, molecular biology and population dynamics)
• Grantee: Fernando Luis Medina Mantelatto
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 18/13685-5 - Integrative analysis of the Brazilian fauna of decapod crustaceans: taxonomy, phylogenetic systematics, spermiotaxonomy, morphology of post-embryonic development, ecology and conservation
• Grantee: Fernando Luis Medina Mantelatto
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants