Male reproductive system and espermiotaxonomy in Dromiidae: there is only one pattern of seminal fluid production, packaging sperm and sperm transfer in crabs?

• Grant number: 16/10394-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2016
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Zoology - Morphology of Recent Groups
• Principal Investigator: Fernando Jose Zara
• Grantee: Maria Alice Garcia Bento
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The Dromiidae family is basal in Brachyura and little known from the histo-morphological and ultrastructural point of view. The general objective will be to detect possible morphological and histological patterns of the male reproductive system, seminal fluid production, mating behavior and sperm transfer. We will also carried out the acrosome reaction and perform espermiotaxonomia in Brazilian Dromiidae, relating with the current literature, in order to understand the evolution of this group in relation to eubrachyuran. Adult individuals will be collected by trawling in Ubatuba, SP. For histological and histochemical description, the reproductive system will be processed according to historesin procedure. For sperm transfer, H. parasitica couples will be kept in aquariums and mate behavior will be filmed and described. The seminal material transfered to the female will be prepared for light microscopy and scanning electron microscopy. For the acrosome reaction steps the samples will be incubated with calcium ionophore A23187 and dimethyl sulfoxide and analysed in interdiferencial contrast microscope, fluorescênia (for cytoskeleton - microtubules) and electronic transmission. The sperm ultrastructure and the acrosome reaction will be processed for routine in Epon-Araldite resin. Since the Dromiidae belong to brachyuran, it is expected that the reproductive system show the standard pattern of regionalization following the usual description to crabs, being the sperm packed in coenospermic spermatophores. Additionally, we expect that H. parasitica shows the acrosome reaction and transfer sperm similar to other brachyuran with espermatheca performing storage site of sperm. The espermiotaxonomy will provide features to elucidate the relationships among the subfamilies of Dromiidae and also to other Podotremata families and superfamilies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA BENTO, MARIA ALICE; LOPEZ GRECO, LAURA S.; ZARA, FERNANDO JOSE. Seminal fluid production and sperm packaging in dromiid crabs (Brachyura, Podotremata). ZOOLOGY, v. 132, p. 17-30, FEB 2019. (16/10394-4, 14/21294-5, 10/50188-8)

GARCIA BENTO, MARIA ALICE; LOPEZ GRECO, LAURA S.; MANTELATTO, FERNANDO L.; ZARA, FERNANDO JOSE. From copula to fertilization: The reproduction of primitive crab Hypoconcha parasitica (Linnaeus, 1763) (Podotremata: Dromiidae). Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. N/A, p. 15-pg., 2022-04-08. (16/10394-4, 10/50188-8, 14/21294-5, 18/13685-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BENTO, Maria Alice Garcia. Male reproductive system and espermiotaxonomy in Dromiidae : there is only one pattern of seminal fluid production, packaging sperm and sperm transfer in crabs?. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.