Oral and Fecal Microbiome in Molar-Incisor Pattern Periodontitis

Author(s)

Amado, Pamela Pontes Penas ^[1] ; Kawamoto, Dione ^[1] ; Albuquerque-Souza, Emmanuel ^[2] ; Franco, Diego Castillo ^{[3, 4]} ; Saraiva, Luciana ^[2] ; Casarin, Renato Correa Viana ^[5] ; Horliana, Anna Carolina Ratto Tempestini ^[6] ; Mayer, Marcia Pinto Alves ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Div Periodontol, Dept Stomatol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Oceanog Inst, Dept Biol Oceanog, Sao Paulo - Brazil

^[4] Jagiellonian Univ, Inst Environm Sci, Fac Biol, Krakow - Poland

^[5] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Prosthodont & Periodont, Sao Paulo - Brazil

^[6] Univ Nove Julho, Biophoton Appl Hlth Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 10, OCT 8 2020.
• Web of Science Citations: 0

Abstract

In order to improve our understanding on the microbial complexity associated with Grade C/molar-incisor pattern periodontitis (GC/MIP), we surveyed the oral and fecal microbiomes of GC/MIP and compared to non-affected individuals (Control). Seven Afro-descendants with GC/MIP and seven age/race/gender-matched controls were evaluated. Biofilms from supra/subgingival sites (OB) and feces were collected and submitted to16S rRNAsequencing.Aggregatibacter actinomycetemcomitans(Aa) JP2 clone genotyping and salivary nitrite levels were determined. Supragingival biofilm of GC/MIP presented greater abundance of opportunistic bacteria.Selenomonaswas increased in subgingival healthy sites of GC/MIP compared to Control.SynergistetesandSpirochaetaewere more abundant whereasActinobacteriawas reduced in OB of GC/MIP compared to controls.Aaabundance was 50 times higher in periodontal sites with PD >= 4 mm of GC/MIP than in controls. GC/MIP oral microbiome was characterized by a reduction in commensals such asKingella, Granulicatella, Haemophilus, Bergeyella, andStreptococcusand enrichment in periodontopathogens, especiallyAaand sulfate reducingDeltaproteobacteria. The oral microbiome of theAaJP2-like+ patient was phylogenetically distant from other GC/MIP individuals. GC/MIP presented a higher abundance of sulfidogenic bacteria in the feces, such asDesulfovibrio fairfieldensis, Erysipelothrix tonsillarum, andPeptostreptococcus anaerobiusthan controls. These preliminary data show that the dysbiosis of the microbiome in Afro-descendants with GC/MIP was not restricted to affected sites, but was also observed in supragingival and subgingival healthy sites, as well as in the feces. The understanding on differences of the microbiome between healthy and GC/MIP patients will help in developing strategies to improve and monitor periodontal treatment. (AU)

• FAPESP's process: 15/00259-0 - Microbial diversity of supra and subgingival biofilms and protein profile of saliva and acquired pellicle of subjects with aggressive periodontitis
• Grantee: Pâmela Pontes Penas Amado
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/14687-6 - Evaluation of immunomodulatory potential of probiotics in periodontal disease
• Grantee: Emmanuel Albuquerque de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/13159-6 - Supra and subgingival microbiota diversity analysis and protein profile of saliva and enamel film of individuals with chronic periodontitis and microbial succession
• Grantee: Dione Kawamoto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/18273-9 - New strategies for the control of periodontitis
• Grantee: Marcia Pinto Alves Mayer
• Support Opportunities: Research Projects - Thematic Grants