Metagenomic and immune-inflammatory analysis of individuals with generalized aggressive periodontitis and their descendants

• Grant number: 16/19970-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): April 01, 2017
• Effective date (End): March 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Renato Corrêa Viana Casarin
• Grantee: Mabelle de Freitas Monteiro
• Supervisor: Purnima Kumar
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Research place: Ohio State University, Columbus, United States
• Associated to the scholarship: 16/03704-7 - Metagenomic and immune-inflammatory analysis of individuals with generalized aggressive periodontitis and their descendants, BP.DR

Abstract

Generalized aggressive periodontitis (GAgP) is a multifactorial disease related to several aspects that influence it installation and progression. The familiar aggregation, an altered inflammatory pattern and the microbial etiology are important characteristic of this disease and could be associated to susceptibility to aggressive periodontitis. Thus, aggressive periodontitis subjects could transmit for their descendants some characteristics, such as susceptibility for colonization and some periodontopathogens, which could influence the development of GAgP. In this vein, the aim of this project is to evaluate the pattern of microbiological colonization as such as the influence of this microbial colonization in the subgingival environment in subjects with GAgP and their children, comparing to periodontally healthy subjects and their children. Thirty families will be selected and divided in two groups: Test group (n=15 families) families in which the parents (or at least one of them) present generalized aggressive periodontitis and one child (age ranging from 6-12 years old); Control group (n=15 families) families in which the parents (both of them) present periodontal healthy and one child (age ranging from 6-12 years old). The groups will be composed using a gender- and age-matched structure. The subgingival biofilm will be collected from first molars and incisors of parents and children and it will be evaluated using a high-throughput technology for microbiological deep sequencing metagenomics analysis. The DNA of biofilm will be extracted and the microbiome and its functional characteristics will be evaluated by metagenomics and bioinformatics analysis. The microbiological data generate in OSU will be evaluated together with the clinical and inflammatory data generate in Brazil. The data will be compared by Chi-square, Student's t test, Mann-Whitney e Wilcoxon tests. The significance level for all analysis will be 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MONTEIRO, MABELLE FREITAS; ALTABTBAEI, KHALED; KUMAR, PURNIMA S.; CASATI, MARCIO ZAFFALON; SILVERIO RUIZ, KARINA GONZALES; SALLUM, ENILSON ANTONIO; NOCITI-JUNIOR, FRANCISCO HUMBERTO; VIANA CASARIN, RENATO CORREA. Parents with periodontitis impact the subgingival colonization of their offspring. SCIENTIFIC REPORTS, v. 11, n. 1, JAN 14 2021. (15/50264-0, 16/03704-7, 16/19970-8)