The Potential of IgG to Induce Murine and Human Thymic Maturation of IL-10+B Cells (B10) Revealed in a Pilot Study

Author(s)

Inoue, Amanda Harumi Sabo ^[1] ; Lira, Aline Aparecida de Lima ^[1] ; De-Oliveira, Marilia Garcia ^[1] ; de Sousa, Thamires Rodrigues ^[1] ; Sgnotto, Fabio da Ressureicao ^[2] ; Duarte, Alberto Jose da Silva ^{[1, 3]} ; Victor, Jefferson Russo ^{[4, 1]}

Affiliation

^[1] Univ Sao Paulo, Lab Med Invest LIM 56, Div Dermatol, Sch Med, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Div Hematol, Sch Med, BR-01246903 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Div Pathol, Sch Med, BR-01246903 Sao Paulo - Brazil

^[4] Laureate Int Univ, FMU, Div Environm Hlth, BR-04505002 Sao Paulo - Brazil

• Document type: Journal article
• Source: CELLS; v. 9, n. 10 OCT 2020.
• Web of Science Citations: 0

Abstract

Regulatory B (B10) cells can control several inflammatory diseases, including allergies; however, the origin of peripheral B10 cells is not fully understood, and the involvement of primary lymphoid organs (PLOs) as a primary site of maturation is not known. Here, using a murine model of allergy inhibition mediated by maternal immunization with ovalbumin (OVA), we aimed to evaluate whether B10 cells can mature in the thymus and whether IgG can mediate this process. Female mice were immunized with OVA, and offspring thymus, bone marrow, spleen, lung, and serum samples were evaluated at different times and after passive transfer of purified IgG or thymocytes. A translational approach was implemented using human nonatopic thymus samples, nonatopic peripheral blood mononuclear cells (PBMCs), and IgG from atopic or nonatopic individuals. Based on the expression of CD1d on B cells during maturation stages, we suggest that B10 cells can also mature in the murine thymus. Murine thymic B10 cells can be induced in vitro and in vivo by IgG and be detected in the spleen and lungs in response to an allergen challenge. Like IgG from atopic individuals, human IgG from nonatopic individuals can induce B10 cells in the infant thymus and adult PBMCs. Our observations suggest that B10 cells may mature in the thymus and that this mechanism may be mediated by IgG in both humans and mice. These observations may support the future development of IgG-based immunoregulatory therapeutic strategies. (AU)

• FAPESP's process: 18/05181-7 - Translational evaluation of IgG effect upon the maturation of offspring thymus derived TCD4, TCD8, nTreg, gammadeltaT and e B cells.
• Grantee: Alberto José da Silva Duarte
• Support Opportunities: Regular Research Grants