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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The aspect ratio of gold nanorods as a cytotoxicity factor on Raphidocelis subcaptata

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Author(s):
Mayrink Nogueira, Patricia Franklin [1] ; Marangoni, Valeria Spolon [1] ; Zucolotto, Valtencir [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Phys Sao Carlos, Nanomed & Nanotoxicol Grp, POB 369, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Environmental Research; v. 191, DEC 2020.
Web of Science Citations: 0
Abstract

Gold nanorods (AuNRs) are promising nanoscale materials for several technological and biomedical applications. The physicochemical properties of AuNRs, including size, shape and surface features, are crucial factors affecting their cytotoxicity. In this study, we investigated the effects of different aspect ratios of AuNRs (1.90, 2.35, 3.25 and 3.50) at concentrations of 2 and 10 mu g mL(-1) on their cytotoxicity and cellular uptake in green algae Raphidocelis subcaptata. The experiment was performed in oligotrophic freshwater medium in a growth chamber with constant agitation of 80 rpm under controlled conditions (120 mu tEm(-2) s(-1) illumination; 12:12h light dark cycle and constant temperature of 22 +/- 2 degrees C). The algal growth was monitored daily for 96 h via electronic absorbance scanning at 600-750 nm. Oxidative stress, cell viability and autofluorescence were evaluated using a flow cytometer. Oxidative stress quantified by loading cultures with the fluorescent dye 2', 7'-dichlomfluorescein diacetate. To assess algal cell viability, propidium iodide was selected as the fluorescent probe. Our results indicated that the aspect ratio of AuNRs mediates their biological effects in green algae R. subcaptata. A positive correlation between oxidative stress and increase of aspect ratio was found at concentration of 10 mu g mL(-1). Higher cytotoxicity and mortality were observed for algae incubated with higher aspect ratios AuNRs (3.50). These findings may be useful to understand the impact of the AuNRs in aquatic environments, contributing to ecosystem management and nanomaterials regulation. (AU)

FAPESP's process: 12/11166-4 - Theranostic nanomaterials applied to the cancer diagnosis and treatment and nanotoxicity studies
Grantee:Valeria Spolon Marangoni
Support Opportunities: Scholarships in Brazil - Doctorate