Isolation, comparison of identification methods and antibiotic resistance of Cronobacter spp. in infant foods

Author(s)

Carvalho, Gabriela Guimaraes ^[1] ; Calarga, Aline Parolin ^[2] ; Teodoro, Josie Roberta ^[1] ; Queiroz, Murilo Mariz ^[1] ; Astudillo-Trujillo, Carlos A. ^[3] ; Levy, Carlos Emilio ^[4] ; Brocchi, Marcelo ^[2] ; Kabuki, Dirce Yorika ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Sch Food Engn, Dept Food Sci, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas, SP - Brazil

^[3] Univ Campinas UNICAMP, Inst Comp, Campinas, SP - Brazil

^[4] Univ Campinas Unicamp, Sch Med Sci, Dept Clin Pathol, Campinas, SP - Brazil

• Document type: Journal article
• Source: Food Research International; v. 137, NOV 2020.
• Web of Science Citations: 0

Abstract

Cronobacter spp. are opportunistic pathogens that cause serious infections, especially in infants, elderly, and immunocompromised people. Dehydrated infant foods are the main vehicle associated with infections caused by these bacteria. Thus, this study aims to investigate the occurrence of Cronobacter spp. in 152 commercial samples of dehydrated infant formulas (77 samples) and dehydrated infant cereals (75 samples), as well as characterize the isolates. Polymerase Chain Reaction (PCR) and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF/MS) methods for isolate identification were used, and their results compared. Furthermore, the susceptibility to 11 antibiotics was tested, and DNA sequencing of one isolate with multi-drug resistance was analyzed. No contamination in the infant formula samples was found, whereas 17.33% (13/75) of the infant cereal samples presented contamination with Cronobacter sakazakii. The identification results by PCR and MALDI-TOF/MS were divergent for some isolates. The antimicrobial resistance results showed a high incidence of resistance to cefazolin (94.4%) besides resistance to amoxicillin (9.45%), cefpodoxime (5.55%), streptomycin (1.35%), and trimethoprim/sulfamethoxazole (1.35%). Whole genome sequencing of one multidrug resistant isolate showed six genes associated with antimicrobial resistance and an 82% possibility of being a human pathogen based on the presence of virulence factors. The presence of Cronobacter spp. in infant foods represents a risk for the infant's health. Moreover, the presence of a pathogenic multi-drug resistant isolate in infant's food reinforces the necessity of improving food safety policies to protect young children. (AU)

• FAPESP's process: 16/14107-0 - Identification and molecular characterization of pathogenic microorganisms in powdered infant products
• Grantee: Dirce Yorika Kabuki
• Support Opportunities: Regular Research Grants