| Full text | |
| Author(s): |
Rossi, Grazielle Guidolin
[1]
;
Guterres, Kevim Bordignon
[1]
;
da Silveira, Carolina Hahn
[2]
;
Moreira, Kelly Schneider
[3]
;
Lima Burgo, Thiago Augusto
[3]
;
Iglesias, Bernardo Almeida
[2]
;
Anraku de Campos, Marli Matiko
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Santa Maria, Lab Mycobacteriol, Santa Maria, RS - Brazil
[2] Univ Fed Santa Maria, Lab Bioinorgan & Porphyrin Mat, Santa Maria, RS - Brazil
[3] Univ Fed Santa Maria, Coulomb Elect & Mechanochem Lab, Av Roraima 1000, Campus Camobi, Santa Maria, RS - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Microbial Pathogenesis; v. 148, NOV 2020. |
| Web of Science Citations: | 3 |
| Abstract | |
In this manuscript, we report, for the first time, the photoinactivation evaluation of tetra-cationic porphyrins with peripheral Pt (II)-bpy complexes in the photodynamic inactivation (PDI) of rapidly growing mycobacterial strains (RGM). Two different isomeric Pt (II)-porphyrins were synthetized and applied. PDI experiments in the strains of Mycobacteroides abscessus subsp. Abscessus (ATCC 19977), Mycolicibacterium fortuitum (ATCC 6841), Mycobacteroides abscessus subsp. Massiliense (ATCC 48898), and Mycolicibacterium smegmatis (ATCC 700084) conducted with adequate concentration (without aggregation) of photosensitizers (PS) under white-light illumination for 90 min showed that the most effective PS significantly reduced the concentration of viable mycobacteria. The present results show that positively charged porphyrins at the meta position (3-PtTPyP) are more efficient PS against M. abscessus, M. fortuitum, M. massiliense, and M. smegmatis. The effectiveness of the molecule as PS for PDI studies is also clear with mycobacteria, which is strongly related with the porphyrin peripheral charge and coordination platinum (II) compounds and consequently their solubility in physiological media. Tetra-cationic PS may be promising anti-mycobacterial PDI agents with potential applications in medical clinical cases and bioremediation. (AU) | |
| FAPESP's process: | 14/50906-9 - INCT 2014: in Functional Complex Materials |
| Grantee: | Fernando Galembeck |
| Support Opportunities: | Research Projects - Thematic Grants |