Sugarcane bagasse hydrolysates as feedstock to produce the isopropanol-butanol-ethanol fuel mixture: Effect of lactic acid derived from microbial contamination on Clostridium beijerinckii DSM 6423

Author(s)

dos Santos Vieira, Carla Ferreira ^[1] ; Codogno, Mateus Cavichioli ^[1] ; Maugeri Filho, Francisco ^[2] ; Maciel Filho, Rubens ^[1] ; Mariano, Adriano Pinto ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Sch Chem Engn, Fermentat Div LOPCA Ferm, Lab Optimizat Design & Adv Control, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Food Engn, Bioproc & Metab Engn Lab LEMeB, Campinas, SP - Brazil

• Document type: Journal article
• Source: Bioresource Technology; v. 319, JAN 2021.
• Web of Science Citations: 6

Abstract

Enzymatic hydrolysis of lignocellulose under industrial conditions is prone to contamination by lactic acid bacteria, and in this study, a cellulose hydrolysate produced from dilute-acid pretreated sugarcane bagasse contained 13 g/L lactic acid and was used for IBE production by Clostridium beijerinckii DSM 6423. In fermentation of the cellulose hydrolysate supplemented with sugarcane molasses for nutrients and buffering of the medium (40 g/L total sugar), 92% of the lactic acid was consumed, and the butanol yield was as high as 0.28 (7.9 g/L butanol), suggesting that lactic acid was preferentially metabolized to butanol. When the hydrolysate was mixed with a detoxified bagasse hemicellulose hydrolysate and supplemented with molasses (35 g/L total sugar), the culture was able to exhaust glucose and utilized sucrose (by 38%), xylose (31%), and lactic acid (70%). Overall, this study shows that C. beijerinckii DSM 6423 can co-ferment firstand second-generation sugars while consuming lactic acid. (AU)

• FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/23983-3 - Routes for production of sugarcane-based tires: efficient conversion of sugars to 1,3-butadiene precursors and techno-economic-environmental assessment
• Grantee: Adriano Pinto Mariano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 16/23042-9 - Development of advanced fermentation technology for the production of n-butanol, isopropanol and ethanol from sugar cane bagasse
• Grantee: Carla Ferreira dos Santos Vieira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/07390-0 - IBE production (isopropanol, butanol, and ethanol) from the fermentation of sugarcane bagasse lignocellulosic sugars mixed with molasses
• Grantee: Mateus Cavichioli Codonho
• Support Opportunities: Scholarships in Brazil - Scientific Initiation