Poly-epsilon-caprolactone Nanoparticles Loaded with 4-Nerolidylcatechol (4-NC) for Growth Inhibition of Microsporum canis

Author(s):Show less -

Greatti, Vanessa Raquel ^[1] ; Oda, Fernando ^[1] ; Sorrechia, Rodrigo ^[1] ; Kapp, Barbara Regina ^[1] ; Seraphim, Carolina Manzato ^[1] ; Villas Boas Weckwerth, Ana Carolina ^[2] ; Chorilli, Marlus ^[1] ; Da Silva, Patricia Bento ^[1] ; Eloy, Josimar O. ^[3] ; Kogan, Marcelo J. ^{[4, 5]} ; Morales, Javier O. ^[4] ; Linhari Rodrigues Pietro, Rosemeire Cristina ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Rodovia Araraquara Jau, Km 1, BR-14800903 Araraquara, SP - Brazil

^[2] Lauro Souza Lima Inst, Dept Mycol, BR-17034971 Bauru, SP - Brazil

^[3] Univ Fed Ceara, Sch Pharm Dent & Nursing, Dept Pharm, Univ Ave, 2853 Rodolfo Te6filo, BR-60430160 Fortaleza, Ceara - Brazil

^[4] Univ Chile, Sch Chem & Pharmaceut Sci, Santos Dumomt 964, Santiago 8380492 - Chile

^[5] Adv Ctr Chron Dis, Sergio Livingstone 1007, Santiago 8380492 - Chile

• Document type: Journal article
• Source: ANTIBIOTICS-BASEL; v. 9, n. 12 DEC 2020.
• Web of Science Citations: 0

Abstract

Dermatophyte fungal infections are difficult to treat because they need long-term treatments. 4-Nerolidylcatechol (4-NC) is a compound found in Piper umbellatum that has been reported to demonstrate significant antifungal activity, but is easily oxidizable. Due to this characteristic, the incorporation in nanostructured systems represents a strategy to guarantee the compound's stability compared to the isolated form and the possibility of improving antifungal activity. The objective of this study was to incorporate 4-NC into polymeric nanoparticles to evaluate, in vitro and in vivo, the growth inhibition of Microsporum canis. 4-NC was isolated from fresh leaves of P. umbellatum, and polymer nanoparticles of polycaprolactone were developed by nanoprecipitation using a 1:5 weight ratio (drug:polymer). Nanoparticles exhibited excellent encapsulation efficiency, and the antifungal activity was observed in nanoparticles with 4-NC incorporated. Polymeric nanoparticles can be a strategy employed for decreased cytotoxicity, increasing the stability and solubility of substances, as well as improving the efficacy of 4-NC. (AU)

• FAPESP's process: 16/02107-5 - Evaluation of antimicrobial potential in vitro and in vivo of Piper umbellatum extracts incorporated or not into nanostructured lipid carriers
• Grantee: Rosemeire Cristina Linhari Rodrigues Pietro
• Support Opportunities: Regular Research Grants