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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hepatic LC3 II/I Ratio Is Not Modulated in Exercised Mice

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Author(s):
Marafon, Bruno Brieda [1] ; Pinto, Ana Paula [2] ; da Rocha, Alisson Luiz [2] ; Rovina, Rafael Lemes [1] ; Pauli, Jose Rodrigo [3] ; De Moura, Leandro Pereira [3] ; Cintra, Dennys Esper [3] ; Ropelle, Eduardo Rochete [3] ; Ramos Da Silva, Adelino Sanchez [1, 2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Physiological Research; v. 69, n. 6, p. 1103-1111, DEC 2020.
Web of Science Citations: 0
Abstract

Autophagy plays an essential role in body homeostasis achievement. One of the main proteins involved in this process is the LC3I, which, after lipidation, leads to the formation of LC3II that participates in the formation and maturation of autophagosome. This descriptive study verified the responses of LC3II to LC3I proteins, as well as the time-course of this ratio in mice livers after different types of acute physical exercise protocols. Eight-week-old male C57BL/6 mice were maintained three per cage with controlled temperature (22 +/- 2 degrees C) on a 12:12-h light-dark normal cycle with food (Purina chow) and water ad libitum. Mice were randomly divided into four groups: control (CT, sedentary mice), resistance (RE, submitted to a single bout of resistance exercise), endurance (EE, submitted to a single bout of endurance exercise), and concurrent (CE, submitted to a single bout of endurance combined with resistance exercise). The mice livers were extracted and used for the immunoblotting technique. The hepatic LC3B II/I ratio for the RE and EE groups were not altered during the different time-points. For the CE group, there was a decrease in this ratio 12h after exercise compared to time 0 and 18h. Also, the hepatic LC3B II/I ratios were not different among the acute physical exercise protocols along the time-course. The hepatic LC3B II/I ratio was not influenced by the endurance and resistance protocols but decreased in response to the concurrent protocol at 12h after the stimulus. (AU)

FAPESP's process: 17/09038-1 - EFFECTS OF REGULAR PHYSICAL EXERCISE AND OVERTRAINING ON THE BEHAVIOR OF THE AUTOPHAGIC PATHWAY IN DIFFERENT TISSUES OF MICE.
Grantee:Adelino Sanchez Ramos da Silva
Support type: Regular Research Grants
FAPESP's process: 19/10875-0 - Effects of regular physical exercise and overtraining on the behavior of the autophagic pathway in different tissues of mice
Grantee:Bruno Brieda Marafon
Support type: Scholarships in Brazil - Technical Training Program - Technical Training