| Full text | |
| Author(s): Show less - |
Abreu, Diego Candido
[1]
;
Gomes, Aline Silva
[1]
;
Tessler, Danielle Klein
[1]
;
Chiebao, Daniela Pontes
[1]
;
Del Fava, Claudia
[1]
;
de Campos Nogueira Romaldini, Adriana Hellmeister
[1]
;
Araujo, Mateus Carvalho
[2]
;
Pompei, Julio
[3]
;
Marques, Guilherme Figueiredo
[3]
;
Harakava, Ricardo
[1]
;
Pituco, Edviges Maristela
[3]
;
de Castro Nassar, Alessandra Figueiredo
[1]
Total Authors: 12
|
| Affiliation: | [1] Ctr Pesquisa Sanidade Anim, Inst Biol, Av Conselheiro Rodrigues Alves 1252, BR-04014002 Sao Paulo, SP - Brazil
[2] Minist Agr Pecuaria & Abastecimento MAPA, Auditora Fiscal Fed Agropecuario, Med Vet, Brasilia, DF - Brazil
[3] Ctr Panamer Febre Aftosa PANAFTOSA OPS OMS, Rio De Janeiro - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | VETERINARY AND ANIMAL SCIENCE; v. 10, DEC 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Glanders is an equine zoonosis caused by Burkholderia mallei that is responsible for considerable economic loss. Complement fixation testing (CFT) using warm or cold incubation are recommended by the OIE, but many routinely used detection tests may present misleading results. To increase accuracy of glanders diagnosis and establish an appropriate protocol in collaboration with the National Equine Health Program, seven horses positive for glanders kept in isolation in Brazil were examined fortnightly by CFT, microbiological screening, and molecular testing. Warm and cold serologies with USDA and c.c.Pro antigens, respectively, were performed on 132 samples using the US Department of Agriculture protocol. The warm and cold serologies showed, respectively,12.9% and 17.3% seroreactive, 85.7% and 65.2% non-reactive, 0.8% and 3% inconclusive, and 0% and 2.3% anticomplementary. The agreement of CFT protocols was moderate. Of 213 clinical samples submitted to selective culture (167 nasal swabs, 5 ocular swabs, 3 lymph node punctures, and 38 tissue samples from four horses that died), 1.9% tested positive for B. mallei. Fourteen samples and one nasal swab (7%) tested positive with PCR. Cold CFT with the USDA and c.c.Pro antigens, in combination with PCR to increase sensitivity, may be useful for diagnosis of chronic glanders. (AU) | |
| FAPESP's process: | 18/08777-8 - Phenotypical and molecular characterization of Bulkholderia mallei in Equidae with glanders |
| Grantee: | Danielle Klein Tessler |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 17/14434-3 - Phenotypical and molecular characterization of Bulkholderia mallei in Equidae with glanders |
| Grantee: | Alessandra Figueiredo de Castro Nassar |
| Support Opportunities: | Regular Research Grants |