3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination

Author(s)

Subramani, Indra Gandi ^[1] ; Perumal, Veeradasan ^{[1, 2]} ; Gopinath, Subash C. B. ^{[3, 4]} ; Mohamed, Norani Muti ^{[1, 5]} ; Joshi, Nirav ^[6] ; Ovinis, Mark ^[2] ; Li Sze, Lim ^[7]

Affiliation

^[1] Univ Teknol Petronas, Ctr Innovat Nanostruct & Nanodevices COINN, Seri Iskandar 32610, Perak - Malaysia

^[2] Univ Teknol Petronas, Mech Engn Dept, Seri Iskandar 32610, Perak - Malaysia

^[3] Inst Nano Elect Engn, Kangar 01000 - Malaysia

^[4] Univ Malaysia Perlis, Sch Bioproc Engn, Arau 02600, Perlis - Malaysia

^[5] Univ Teknol Petronas, Dept Fundamental & Appl Sci, Seri Iskandar 32610, Perak - Malaysia

^[6] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

^[7] Med Innovat Ventures Sdn Bhd Mediven, Gelugor 11700, Penang - Malaysia

• Document type: Journal article
• Source: Journal of the Taiwan Institute of Chemical Engineers; v. 116, p. 26-35, NOV 2020.
• Web of Science Citations: 0

Abstract

Recently, non-faradaic electrochemical impedimetric (nfEIS) has been identified as a dynamic and robust method for bio-determining. However, the success of a nfEIS approach largely depends on its sensing layer architecture. In this study, the use of a Glutaraldehyde (GA) crosslinked copper (Cu) - bovine serum albumin (BSA) hybrid nanoflower with three-dimensional nanoporous architecture for the sensing layer was investigated. The nanoflower morphology changes were observed under FESEM, revealing loosely interlaced nanoflower into a tightly interlaced, highly porous structure upon GA crosslinking. This nanoflower was hybridized to immobilize aminated-DNA probe on the transducer surface and detect the target TB DNA in their natural redox-free environment. FTIR and XPS characterization showed distinct peaks at 950-1100 cm(-1) (P-O, P=O bonds from nanoflower and DNA backbone) and 286.48 eV (interaction between BSA and aminated DNA), respectively, validating the successful DNA probe immobilization on the nanoflower surface. Furthermore, impedimetric sensing in a redox-free environment showed that the developed TB biosensor present has a detection limit (LOD) of 60 pM with a (linear) range from 1 pM to 1 mu M with good reproducibility. This redox-free non-faradaic EIS offers excellent biosensing potential and may be extended for diagnos ing other biomarkers in clinical practice. (c) 2020 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 14/23546-1 - Enhanced sensitive and selective sensors made with p-NiO nanowire/n-ZnO Nanowire heterostructures
• Grantee: Niravkumar Jitendrabhai Joshi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/23474-6 - Nanomaterial-based heterostructures: synthesis and their gas sensing properties
• Grantee: Niravkumar Jitendrabhai Joshi
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor