Comparative eye and liver differentially expressed genes reveal monochromatic vision and cancer resistance in the shortfin mako shark (Isurus oxyrinchus)

Author(s)

Domingues, Rodrigo R. ^{[1, 2, 3]} ; Mastrochirico-Filho, Vito Antonio ^[4] ; Mendes, Natalia J. ^[4] ; Hashimoto, Diogo T. ^[4] ; Coelho, Rui ^{[5, 6]} ; da Cruz, Vanessa Paes ^[7] ; Antunes, Agostinho ^{[1, 3]} ; Foresti, Fausto ^[7] ; Mendonca, Fernando F. ^[2]

Affiliation

^[1] Univ Porto, Fac Ciencias, Dept Biol, Rua Campo Alegre S-N, P-4169007 Porto - Portugal

^[2] Univ Fed Sao Paulo UNIFESP, Inst Mar, Dept Ciencias Mar, Rua Carvalho de Mendonca 144, BR-11070100 Sao Paulo - Brazil

^[3] Univ Porto, Ctr Interdisciplinar Invest Marinha & Ambiental, CIIMAR CIMAR, Av Gen Norton Matos S-N, P-4450208 Porto - Portugal

^[4] Univ Estadual Paulista UNESP, Ctr Aquicultura UNESP, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Sao Paulo - Brazil

^[5] Inst Portugues Mar & Atmosfera IPMA, IP, Av 5 Outubro, P-8700305 Olhao - Portugal

^[6] Univ Algarve, Ctr Ciencias Mar CCMAR, Campus Gambelas, P-8005139 Faro - Portugal

^[7] Univ Estadual Paulista UNESP, Inst Biociencias Botucatu, Lab Biol & Genet Peixes, BR-18618689 Botucatu, SP - Brazil

• Document type: Journal article
• Source: Genomics; v. 112, n. 6, p. 4817-4826, NOV 2020.
• Web of Science Citations: 0

Abstract

The shortfin mako, Isurus oxyrinchus is an oceanic pelagic shark found worldwide in tropical and subtropical waters. However, the understanding of its biology at molecular level is still incipient. We sequenced the messenger RNA isolated from eye and liver tissues. De novo transcriptome yielded a total of 705,940 transcripts. A total of 3774 genes were differentially expressed (DEGs), with 1612 in the eye and 2162 in the liver. Most DEGs in the eye were related to structural and signaling functions, including nonocular and ocular opsin genes, whereas nine out of ten most overexpressed genes in the liver were related to tumor suppression, wound healing, and human diseases. Furthermore, DEGs findings provide insights on the monochromatic shark vision and a repertory of cancer-related genes, which may be insightful to elucidate shark resistance to cancer. Therefore, our results provide valuable sequence resources for future functional and population studies. (AU)

• FAPESP's process: 17/02420-8 - High-tech conservation: combining oceanography, transcriptome and genomics to assess population genetic connectivity and local adaptation of the highly migratory Mako shark (Isurus oxyrinchus) in the Atlantic Ocean
• Grantee: Rodrigo Rodrigues Domingues
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/19740-7 - A wide scale inter-hemispheric and inter-disciplinary study aiming the conservation of the shortfin mako shark in the Atlantic Ocean
• Grantee: Fausto Foresti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/21319-9 - Use of omics and bioinformatics tools for detect local adaptation of the shortfin mako shark (Isurus oxyrinchus) in the Atlantic and Indian Ocean
• Grantee: Rodrigo Rodrigues Domingues
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor