Expression analysis of genes regulated by protein Dermicidina in malignant melanoma cells G-361 by DNA microarray

• Grant number: 12/02497-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2012
• End date: April 30, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: José Ernesto Belizario
• Grantee: Nancy Marcela Pérez Sosa
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The protein dermicidina (DCD) is encoded by a gene localized in chromosome 12q13.1 portion 12, present only in primates and humans. The protein is secreted by exocrine cells of the skin melanocytes, neurons and epithelial cells of normal breast. Some studies have shown the importance of protein in oncogenic processes in DCD with breast, prostate and melanoma, revealed by its role as a growth factor and cell survival. In studies performed in our laboratory showed that the DCD is expressed in normal skin cells, placenta, brain and in various tumors, including breast carcinomas and malignant melanoma. Biological and biochemical tests showed that the "knockdown" in the expression of DCD in malignant melanoma via G-361 RNA interference (RNAi) significantly decreased the growth in vitro in cell culture and tumor formation in nude mice. Similar results were obtained when nude mice transplanted with cells of G-361 melanoma were treated with rabbit polyclonal antibodies against the protein DCD, administered once a week for 5 weeks. In the present project aims to identify genes differentially expressed in cells of malignant melanoma G-361 control and RNAi cells expressing DCD to analyzing the global gene expression by DNA microarray technology (Human GeneChip 1.0 ST array). Genes differentially expressed (> 3-fold) between the control and expressing RNAi will be validated by testing expression by real-time PCR, immunohistochemistry, Western blot and indirect immunofluorescence. We also intend to make assessments of functional intracellular signaling pathways of proliferation and resistance to cell death. It is expected from these studies show that the protein acts as an oncogene DCD promoting the transcription of genes involved in transformation of normal melanocytes and progression to malignant melanocytes.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BELIZARIO, JOSE E.; SIRCILI, MARCELO P.. Novel biotechnological approaches for monitoring and immunization against resistant to antibiotics Escherichia coli and other pathogenic bacteria. BMC Veterinary Research, v. 16, n. 1, NOV 2 2020. (12/02497-7, 14/20847-0, 10/06707-0, 08/05519-6)

BELIZARIO, JOSE E.. Cancer Risks Linked to the Bad Luck Hypothesis and Epigenomic Mutational Signatures. EPIGENOMES, v. 2, n. 3, SEP 2018. (12/02497-7, 14/20847-0)

BELIZARIO, JOSE E.; SANGIULIANO, BEATRIZ A.; VIANA-SANTOS, BEATRIZ; PEREZ-SOSA, MARCELA; CALDEIRA, IZABELA. Microscopes and technologies for imaging cells and their protein networks: From nano to atomic scale resolution. TECHNOLOGY, v. 5, n. 2, p. 61-73, JUN 2017. (12/02497-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOSA, Nancy Marcela Pérez. Gene expression analysis regulated by Dermcidin protein in G-361 malignant melanoma cell line by DNA-microarray. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.