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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Altered shear stress on endothelial cells leads to remodeling of extracellular matrix and induction of angiogenesis

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Author(s):
Russo, T. A. [1] ; Banuth, A. M. M. [1] ; Nader, H. B. [1] ; Dreyfuss, J. L. [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Paulo, Escola Paulista Med, Carl Peter von Dietrich Lab, Mol Biol Div, Dept Biochem, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PLoS One; v. 15, n. 11 NOV 19 2020.
Web of Science Citations: 0
Abstract

Endothelial cells (ECs) are subjected to physical forces such as shear stress (SS) induced by blood flow that leads to significant changes in morphology, physiology and gene expression. The abnormal mechanical forces applied in the cardiovascular system can influence the development of conditions and diseases such as thrombosis, hypertension and atherosclerosis. This study investigated the expression of glycosaminoglycans (GAGs), proteoglycans and extracellular matrix molecules in ECs exposed to normal and altered SS. ECs were exposed to SS of 12 dyn/cm(2) (artery physiological condition) and 4 dyn/cm(2) (artery pathological condition). Subsequently, ECs were subjected to immunofluorescence, qPCR, GAG biosynthesis analyses and cell-based assays. SS induced changes in ECs morphology. There were other pathological consequences of altered SS, including inhibited adhesion, stimulation of migration and capillary-like tube formation, as well as increases of GAG synthesis. We observed higher expression of syndecan-4, perlecan, decorin, fibronectin and collagen III alpha 1 and growth factors, including VEGF-A and TGF beta-1. ECs exposed to SS displayed extracellular matrix remodeling as well as expression of cell-matrix and cell-cell interaction molecules. This study contributes to the understanding of how vascular biology is affected by mechanical forces and how these molecules can be affected in cardiovascular diseases. (AU)

FAPESP's process: 14/10156-0 - Role of extracellular matrix components in mechanotransduction of endothelial cells
Grantee:Juliana Luporini Dreyfuss Regatieri
Support Opportunities: Regular Research Grants