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Endothelial to mesenchymal transition during vein arterialization remodeling: the role of mechanical stretch on endothelial plasticity

Grant number: 14/06844-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2014
Effective date (End): August 31, 2018
Field of knowledge:Health Sciences - Medicine
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Ayumi Aurea Miyakawa Yamaguchi
Grantee:Thais Girão da Silva
Home Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:13/17368-0 - Cardiovascular genomics: mechanisms & novel therapeutics - CVGen mech2ther, AP.TEM


Saphenous vein graft is widely used for cardiac revascularization in ischemic myocardium. However, adaptive response to the new hemodynamic condition predisposes the vein graft to occlusion due to the remodeling with increased extracellular matrix and cells expressing ±-SMA. Experimental evidence demonstrates that endothelial to mesenchymal transition (EndMT) is one source of ±-SMA positive cells during vascular remodeling. EndMT is characterized by endothelial markers reduction, gain of mesenchymal markers and increased deposition of matrix. This process is dependent on TGF² signaling and recently it was demonstrated that combined stimulation of IL1b + TGFb2 enhances this phenotypic transition in vitro. Vein grafts are submitted to changes in hemodynamic forces, such as increased shear stress and cyclic strain. It is demonstrated that endothelial cells subjected to laminar shear stress are resistant to EndMT induction and there is lack of information regarding to stretch influence in this process. The hypothesis of the present work is that mechanical stretch induces and/or enhances the EndMT process in a pro-inflammatory (IL1b) and pro-fibrotic environment (TGFb). Data from our laboratory shows that IL1b is increased during vein graft arterialization remodeling and we want to associate with mechanical stimulus to understand the contribution of EndMT to the pathological vascular remodeling. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MIYAKAWA, AYUMI A.; GIRAO-SILVA, THAIS; KRIEGER, JOSE E.; EDELMAN, ELAZER R. Rapamycin activates TGF receptor independently of its ligand: implications for endothelial dysfunction. Clinical Science, v. 132, n. 4, p. 437-447, FEB 28 2018. Web of Science Citations: 2.

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