Methanotrophic Community Detected by DNA-SIP at Bertioga's Mangrove Area, Southeast Brazil

Author(s)

do Carmo Linhares, Debora ^{[1, 2]} ; Saia, Flavia Talarico ^[3] ; Duarte, Rubens Tadeu Delgado ^[4] ; Nakayama, Cristina Rossi ^[5] ; de Melo, Itamar Soares ^[6] ; Pellizari, Vivian Helena ^[2]

Affiliation

^[1] Inst Technol Res Sao Paulo, Lab Ind Biotechnol, BR-05508901 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Oceanog Inst, Dept Biol Oceanog, Praca Oceanog 191, BR-05508120 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Inst Marine Sci, Ave Dr Carvalho de Mendonca, 144, Encruzilhada, BR-11070102 Santos, SP - Brazil

^[4] Univ Fed Santa Catarina, Dept Microbiol Immunol & Parasitol, Lab Mol Ecol & Extremophiles, BR-88040900 Florianopolis, SC - Brazil

^[5] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Rua Sao Nicolau 210, BR-09913030 Diadema, SP - Brazil

^[6] EMBRAPA Environm, Environm Microbiol Lab, Jaguariuna, SP - Brazil

• Document type: Journal article
• Source: MICROBIAL ECOLOGY; v. 81, n. 4 JAN 2021.
• Web of Science Citations: 0

Abstract

Methanotrophic bacteria can use methane as sole carbon and energy source. Its importance in the environment is related to the mitigation of methane emissions from soil and water to the atmosphere. Brazilian mangroves are highly productive, have potential to methane production, and it is inferred that methanotrophic community is of great importance for this ecosystem. The scope of this study was to investigate the functional and taxonomic diversity of methanotrophic bacteria present in the anthropogenic impacted sediments from Bertioga ` s mangrove (SP, Brazil). Sediment sample was cultivated with methane and the microbiota actively involved in methane oxidation was identified by DNA-based stable isotope probing (DNA-SIP) using methane as a labeled substrate. After 4 days (96 h) of incubation and consumption of 0.7 mmol of methane, the most active microorganisms were related to methanotrophs Methylomonas and Methylobacter as well as to methylotrophic Methylotenera, indicating a possible association of these bacterial groups within a methane-derived food chain in the Bertioga mangrove. The abundance of genera Methylomonas, able to couple methane oxidation to nitrate reduction, may indicate that under low dissolved oxygen tensions, some aerobic methanotrophs could shift to intraerobic methane oxidation to avoid oxygen starvation. (AU)

• FAPESP's process: 04/13910-6 - Biodiversity and functional activities of microorganisms from mangrove of the State of São Paulo
• Grantee: Itamar Soares de Melo
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 09/06601-0 - Characterization of methanotrophic community and its isolates from Bertioga´s mangroves, São Paulo
• Grantee: Débora Do Carmo Linhares
• Support Opportunities: Scholarships in Brazil - Master