| Full text | |
| Author(s): |
de Melo, Ricardo Rodrigues
[1]
;
de Lima, Evandro Antonio
[1]
;
Persinoti, Gabriela Felix
[1]
;
Vieira, Plinio Salmazo
[1]
;
de Sousa, Amanda Silva
[1]
;
Zanphorlin, Leticia Maria
[1]
;
de Giuseppe, Priscila Oliveira
[1]
;
Ruller, Roberto
[2]
;
Murakami, Mario Tyago
[1]
Total Authors: 9
|
| Affiliation: | [1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil
[2] Fed Univ Mato Grosso do Sul UFMS, Inst Biosci INBio, Microorganisms & Gen Biochem Lab, Campo Grande, MS - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 166, p. 190-199, JAN 1 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Cold-adapted endo-beta-1,4-glucanases hold great potential for industrial processes requiring high activity at mild temperatures such as in food processing and extraction of bioactive compounds from plants. Here, we identified and explored the specificity, mode of action, kinetic behavior, molecular structure and biotechnological application of a novel endo-beta-1,4-glucanase (XacCel8) from the phytopathogen Xanthomonas citri subsp. citri. This enzyme belongs to an uncharacterized phylogenetic branch of the glycoside hydrolase family 8 (GH8) and specifically deaves internal beta-1.4-linkages of cellulose and mixed-linkage beta-glucans releasing short cellooligosaccharides ranging from cellobiose to cellohexaose. XacCel8 acts in near-neutral pHs and in a broad temperature range (10-50 degrees C), which are distinguishing features from conventional thermophilic beta-1,4-glucanases. Interestingly, XacCel8 was greatly stimulated by cobalt ions, which conferred higher conformational stability and boosted the enzyme turnover number. The potential application of XacCel8 was demonstrated in the caffeine extraction from guarana seeds, which improved the yield by 25 g/kg compared to the traditional hydroethanolic method (HEM), indicating to be an effective additive in this industrial process. Therefore, XacCel8 is a metalstimulated and cold-adapted endo-beta-1,4-glucanase that could be applied in a diverse range of biotechnological processes under mild conditions such as caffeine extraction from guarana seeds. (C) 2020 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 15/26982-0 - Exploring novel strategies for depolymerization of plant cell-wall polysaccharides: from structure, function and rational design of glycosyl hydrolases to biological implications and potential biotechnological applications |
| Grantee: | Mário Tyago Murakami |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/14253-9 - Evaluation of the biotechnological potential of the cellulolytic system of Xanthomonas axonopodis pv. citri: a structural, functional and applied approach |
| Grantee: | Ricardo Rodrigues de Melo |
| Support type: | Scholarships in Brazil - Post-Doctorate |