Comparison of plasma and intra-articular gentamicin levels after intravenous administration in healthy horses

Author(s)

Ferreira, Marilia Alves ^[1] ; Aguiar, Mayra Lissoni ^[1] ; Grigoletto, Renan ^[1] ; Passarelli, Danielle ^[1] ; Arantes, Julia de Assis ^[1] ; Reginato, Gustavo Morandini ^[1] ; Salles Brito, Pedro Henrique ^[1] ; Fernandes da Silva, Thiago Jonatha ^[1] ; Sampaio Doria, Renata Gebara ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, Pirassununga, SP - Brazil

• Document type: Journal article
• Source: ACTA VETERINARIA BRNO; v. 89, n. 3, p. 225-230, 2020.
• Web of Science Citations: 0

Abstract

Septic arthritis is considered one of the most severe disorders that affect horses' joints. Therapy is intended to eliminate the microorganisms that cause this infection. The intravenous administration of antibiotics is an easily performed technique, but there is no guarantee that the antibiotics will reach the joint milieu at high concentrations and remain elevated for a certain period of time. This study aimed to determine and compare the levels of gentamicin in the plasma and synovial fluid of healthy horses after intravenous administration for seven days. Five horses received 6.6 mg/kg intravenous gentamicin every 24 h for seven consecutive days. Blood and synovial fluid samples were collected from the right metacarpal-phalangeal joint before the start of the treatment (T0) and after 6 h (T6).. 12 h (F12). and every 24 h during antibiotic therapy for seven days (D1 to D7). The levels of gentamicin in scrum and synovial fluid were quantified by Enzyme-Linked Immunosorbent Assay (ELISA). The intravenous administration of 6.6 mg/kg of gentainicin, in horses, after a period of 24 h, promotes synovial fluid concentrations below the MIC of the drug. in spite of reaching high plasma concentrations of gentamicin, daily mean serum concentration which was 29-fold higher than the minimum inhibitory concentration (MIC) of the drug. The intravenous administration every 24 h during seven consecutive days did not cause systemic side effects, such as hepatic or renal injury. (AU)

• FAPESP's process: 19/03193-0 - Transient response of inflammatory mediators induced by lipopolysaccharide in blood and allantoic fluid of pregnant mares.
• Grantee: Renata Gebara Sampaio Dória
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/20662-8 - Avaliação da concentração intra-articular de gentamicina administrada por via intravenosa em membros de equinos sadios
• Grantee: Mayra Lissoni Aguiar
• Support Opportunities: Scholarships in Brazil - Scientific Initiation