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Transient response of inflammatory mediators induced by lipopolysaccharide in blood and allantoic fluid of pregnant mares

Grant number: 19/03193-0
Support type:Regular Research Grants
Duration: July 01, 2019 - June 30, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Renata Gebara Sampaio Dória
Grantee:Renata Gebara Sampaio Dória
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Assoc. researchers:Adriana Helena de Souza ; Carlos Augusto Araújo Valadão ; Fernando de Queiroz Cunha ; Juliana Regina Peiró ; Luciano Andrade Silva


Endotoxemia is a frequent consequence of gastrointestinal diseases in horses and leads to the activation of the inflammatory cascade, including the release of proinflammatory cytokines, from pathogen recognition and pathogen-associated molecular patterns (PAMPS), through (PRR), which detect a specific ligand, such as lipopolysaccharide (LPS), the main endotoxin related to this inflammatory condition in horses. Studies have reported effects of maternal colic on the equine fetus and associated abortions with signs of endotoxemia in mares in late gestation, suggesting the influence of this systemic syndrome on the fetus. Elevated levels of endotoxins and inflammatory cytokines have been demonstrated in the amniotic fluid of women with membrane rupture and preterm labor. The fetoplacental unit is a dynamic system of exchange between maternal and fetal fluids. In human medicine, amniotic fluid evaluation is widely used to analyze fetal viability. However, in equines, the physiology of fetal fluids and their chemical composition is poorly understood and few studies have been developed for this purpose, especially with diseased pregnant mares. This work aims to determine and quantify inflammatory mediators, nuclear factor NFº-B activity and gene expression of the TLR-4 receptor in the blood and fetal fluids of mares in late gestation during maternal systemic endotoxemia induced by intravenous infusion of LPS, in order to understand the pathophysiology of endotoxemia in pregnant mares and their influence on the fetus. (AU)