Electrical stimulation enhances early palatal wound healing in mice

Author(s):Show less -

Ferreira, Camila Lopes ^[1] ; Neves Jardini, Maria Aparecida ^[2] ; Moretto Nunes, Camilla Magnoni ^[1] ; Bernardo, Daniella Vicensotto ^[1] ; Viana Casarin, Renato Correa ^[3] ; Gedraite, Estevao dos Santos ^[4] ; Mathias, Marcio Antonio ^[4] ; Liu, Fei ^[5] ; Mendonca, Gustavo ^[5] ; Silveira Mendonca, Daniela Baccelli ^[5] ; Santamaria, Mauro Pedrine ^[2]

Affiliation

^[1] Sao Paulo State Univ UNESP, Coll Dent, Periodont Area, Oral Biopathol Program, Sao Jose Dos Campos - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Sci & Technol, Div Periodont, Av Eng Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Campinas UNICAMP, Dept Prosthodont & Periodont, Div Periodont, Piracicaba Dent Sch, Piracicaba, SP - Brazil

^[4] FEI Coll Ind Engn, Sao Bernardo Do Campo, SP - Brazil

^[5] Univ Michigan, Sch Dent, Dept Biol & Mat Sci & Prosthodont, Ann Arbor, MI 48109 - USA

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 122, FEB 2021.
• Web of Science Citations: 0

Abstract

Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 mu A; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1 beta, IL-6, IL-10 TNF-alpha, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-alpha, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phosphoSmad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers. (AU)

• FAPESP's process: 16/07528-9 - Electrical stimulation effect on coronally advanced flap for the treatment of gingival recession: randomized controlled clinical trial
• Grantee: Felipe Lucas da Silva Neves
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/16102-5 - Periodontal plastic surgery: assessment of periodontal wound healing immunology and randomized clinical trial for the development of new treatment protocol
• Grantee: Mauro Pedrine Santamaria
• Support Opportunities: Regular Research Grants