Evaluation of the aesthetic effect, enamel microhardness and trans-amelodentinal cytotoxicity of a new bleaching agent for professional use containing trimetaphosphate and fluoride

Author(s)

Felipe Akabane, Sara Tiemi ^[1] ; Danelon, Marcelle ^{[2, 1]} ; Nunes, Gabriel Pereira ^[1] ; Gruba, Amanda Scarpin ^[1] ; de Souza-Costa, Carlos Alberto ^[3] ; de Oliveira Duque, Carla Caroline ^[4] ; Gallinari, Marjorie de Oliveira ^[1] ; Fraga Briso, Andre Luiz ^[1] ; Botazzo Delbem, Alberto Carlos ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Aracatuba Sch Dent, Dept Prevent & Restorat Dent, Rua Jose Bonifacio, 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Univ Ribeirao Preto UNAERP, Sch Dent, Ribeirao Preto, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 114, FEB 2021.
• Web of Science Citations: 0

Abstract

This study evaluated the effects of calcium gluconate (CaGlu), sodium fluoride (NaF), sodium trimetaphosphate (TMP), and NaF/TMP when added to a 35% hydrogen peroxide (H2O2) bleaching gel on the color change, enamel hardness, penetration and cytotoxicity trans-amelodentinal. Bovine enamel/dentin disks (n = 288) were divided according to the bleaching gel: 35% H2O2; 35% H2O2 + 0.05% NaF; 35% H2O2 + 0.25% TMP; 35% H2O2 + 0.05% NaF + 0.25% TMP; 35% H2O2 + 0.1% NaF + 1% TMP and 35% H2O2 + 2% CaGlu. The bleaching gels were applied thrice (40 min/session) at 7-day intervals between each application. Then, the color change, percentage of surface hardness loss (%511), cross-sectional hardness (Delta KHN), trans-amelodentinal penetration of H2O2, cell viability and morphology (MDPC-23 odontoblast-like cells), alkaline phosphatase activity (ALP) and deposition of mineralization nodules were determined. The data were submitted to ANOVA, followed by the Student-Newman-Keuls test (p < 0.05). All bleaching gels showed significant color changes after treatment (p < 0.001). Mineral loss (%SH and Delta KHN) and H2O2 penetration were lower for 35% H2O2/0.1% NaF/1% TMP; 35% H2O2/2% CaGlu, meanwhile, showed higher values, compared to the other groups (p < 0.001). Cell viability was around 9%, except for the bleaching gel containing 35% H2O2/0.1% NaF/1% TMP with 12.8% (p < 0.05). ALP was higher for groups containing TMP compared to other whitening gels (p < 0.05). The formation of mineralization nodules was greater for gels containing NaF/TMP or CaGlu (p < 0.05). The alterations of cell morphology were intense for all bleaching gels. It was concluded that the addition of NaF/TMP in-office bleaching did not interfere in bleaching efficacy, reduced enamel demineralization, H2O2 penetration and cytotoxicity. (AU)

• FAPESP's process: 18/08769-5 - Evaluation of a new bleaching agent formulation containing sodium trimetaphosphate on color change, trans-amelodentinium penetration of hydrogen peroxide, cytotoxicity, microhardness and heat generation: in vitro study
• Grantee: Sara Tiemi Felipe Akabane
• Support Opportunities: Scholarships in Brazil - Master