Performance of MDP-based system in eroded and carious dentin associated with proteolytic inhibitors: 18-Month exploratory study

Author(s)

Giacomini, Marina Ciccone ^{[1, 2]} ; Candia Scaffa, Polliana Mendes ^[3] ; Goncalves, Rafael Simoes ^[1] ; Jacomine, Juliana Carvalho ^[1] ; Zabeu, Giovanna Speranza ^{[1, 4]} ; de Oliveira Carrilho, Marcela Rocha ^[5] ; Honorio, Heitor Marques ^[6] ; Wang, Linda ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Operat Dent Endodont & Dent Mat, Bauru Sch Dent, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Ctr Integrated Faculties Ourinhos, BR 153, Km 338 S-N Agua Cateto, BR-19909100 Ourinhos, SP - Brazil

^[3] Oregon Hlth & Sci Univ, OHSU, Dept Biomat & Biomech, 2730 SW Moody Ave, Portland, OR 97201 - USA

^[4] Ctr Univ Sagrado Coracao UNISAGRADO, Sch Dent, Rua Irma Arminda 10-50, BR-17011160 Bauru, SP - Brazil

^[5] Midwestern Univ, Coll Dent Med Illinois, 55531st St, Downers Grove, IL 60515 - USA

^[6] Univ Sao Paulo, Baum Sch Dent, Dept Pediat Dent Orthodont & Collect Hlth, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 114, FEB 2021.
• Web of Science Citations: 0

Abstract

The aim of this study was to explore the impact of the interaction between an MDP-based universal adhesive system in etch-and-rinse mode and two proteolytic inhibitors on the longevity of restorations bonded to artificially-affected-dentin substrates. 90 sound human third molars were randomly distributed into three groups according to the substrate: N-no challenges-control (stored in artificial saliva), ACD-artificial caries dentin (6 h DE + 18 h-RE/5 days + 48 h RE) and ERO-artificial erosion dentin (3 x 5 min/5 days with orange juice). They were further redistributed according to dentin pretreatment: Wwater (control), CHX-2% digluconate chlorhexidine and E645 mu M E64-Trans-Epoxysuccinyl-L-Leucylamido-(4-guanidino) butane, which resulted in the following 9 groups (n = 10): N-W, N-CHX, N-E64, ACD-W, ACD-CHX, ACD-E64, ERO-W, ERO-CHX and EROE64. All specimens were restored with Adper Single Bond Universal (Etch-and-rinse mode)/Filtek Z250. Sticks (0.64 mm(2)) were obtained and subjected to microtensile test (mu TBS) in a universal testing machine at 0.5 mm/ min for 7-days, 6 and 18-month analyses. Failure modes were classified using optical microscopy (40X). Data were statistically analyzed by three-way ANOVA and Tukey tests (p < 0.05). All individual factors (p < 0.0001) and interaction between factors were statistically significant (substrate X pretreatment (p = 0.00093); substrate X time (p = 0.01035) and pretreatment X time (p = 0.0035). Caries-affected substrate was the most compromised one, disregarding the pretreatment. CHX was mostly affected compared with E64 up to 18 months, possibly due to its calcium-dependent mechanism. (AU)

• FAPESP's process: 13/12203-3 - Effect of protelolytic inhibitors on bonding to artificial carious or eroded demineralized dentin
• Grantee: Marina Ciccone Giacomini Belei
• Support Opportunities: Scholarships in Brazil - Master