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Effect of protelolytic inhibitors on bonding to artificial carious or eroded demineralized dentin

Grant number: 13/12203-3
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2014
Effective date (End): February 28, 2015
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Linda Wang
Grantee:Marina Ciccone Giacomini Belei
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


In restorative treatment, bonding procedures represent essential part for the establishment of hybrid layer, whose longevity depends on factors related to the properties of employed materials and their interaction with dental substrates overtime. Studies have shown that dentin intrinsic proteolytic enzymes such as matrix metalloproteinases (MMPs) and cysteine cathepsins present in the dentin-pulp complex can be reactivated by the low pH in situations as dental caries or erosive process. The aim of this study is to evaluate the effect of an universal adhesive system (etch-and-rinse mode) on bonding to dentin in different conditions (artificial caries or erosion) and pretreated with proteolytic inhibitors. Ninety molars will be selected and prepared as flat surfaces treated with 600 sandpaper for 1 minute. The specimens will be randomly divided into three groups according to the substrate: CONT- with no simulations, CA- artificial caries simulation, E-artificial erosion simulation. Then, each of these groups will be redivided into three subgroups according to the pretreatment of dentin: A- treated with water; CHX-treated with chlorhexidine and E64- treated with E64 inhibitor. Therefore, they will consitute 9 groups (n = 10): CONT-A, CONT-CHX, CONT-E64, CA-A, CA-CHX, CA-E64, E-A, E-CHX, E-E64. All specimens will be restored with Single Bond ® Universal and composite resin Filtek Z250. The teeth will be cut to obtain sticks (area of approximately 0.64 mm2), which will be subjected to the test in the testing machine at 0.5 mm/min. The mode failures will be classified according to the analysis of interfaces by optical microscopy 40X. Data will be tested to verify normal distribution and homogeneity to be analyzed by ANOVA and multiple comparison tests (p <0.05).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GIACOMINI, M. C.; SCAFFA, P. M. C.; CHAVES, L. P.; VIDAL, C. M. P.; MACHADO, T. N.; HONORIO, H. M.; TJADERHANE, L.; WANG, L. Role of Proteolytic Enzyme Inhibitors on Carious and Eroded Dentin Associated With a Universal Bonding System. Operative Dentistry, v. 42, n. 6, p. E188-E196, NOV-DEC 2017. Web of Science Citations: 5.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BELEI, Marina Ciccone Giacomini. Effect of proteolytic inhibitors in carious and eroded dentin adhesion. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru Bauru.

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