| Full text | |
| Author(s): |
Leal, Camila Rabelo Oliveira
[1]
;
Jorge, Leonardo Re
[2]
;
de Souza, Anete Pereira
[3]
;
Lewinsohn, Thomas Michael
[1]
;
Silva-Brandao, Karina Lucas
[4]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Dept Biol Anim, Inst Biol, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] Czech Acad Sci, Biol Ctr, Inst Entomol, Dept Ecol, Branisovska 31, Ceske Budejovice 37005 - Czech Republic
[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Av Candido Rondom 400, BR-13083875 Campinas, SP - Brazil
[4] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | APPLIED ENTOMOLOGY AND ZOOLOGY; v. 56, n. 1, p. 125-132, FEB 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Tomoplagia (Diptera: Tephritidae) are endophagous herbivores on flowerheads of Asteraceae, with varying levels of host specialization. We isolated, characterized, and tested the heterologous amplification for sets of microsatellites of three species from this genus associated with native hosts in the Espinhaco mountain range, Brazil. We used two protocols to build microsatellite-enriched libraries and characterized 12 polymorphic loci for each fruit-fly species. The samples of T. grandis, a monophagous species, presented a lower number of alleles than the samples of the oligophagous species T. incompleta and T. bicolor. The average observed heterozygosity did not vary among species and the high values of Polymorphism Information Content suggest that the characterized microsatellite loci are quite informative. The primers developed for each Tomoplagia species failed to amplify the microsatellite loci for the other two species, which indicates a low conservation of the flanking nucleotide sequences in the three evaluated species. The sets of polymorphic microsatellites characterized for these species of Tomoplagia may be used in future ecological and evolutionary studies that aim to detect patterns of diversity on a fine scale. (AU) | |
| FAPESP's process: | 12/16266-7 - Population genomics: a new approach for studies of speciation in insects due to host use applied to the development of sustainable strategies of IPM |
| Grantee: | Karina Lucas da Silva-Brandão |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/10236-7 - Trophic specialization and genetic structure in phytophagous insects |
| Grantee: | Camila Rabelo Oliveira Leal |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/24821-1 - Genetic diversity and interaction structure of insects and plants |
| Grantee: | Thomas Michael Lewinsohn |
| Support Opportunities: | Regular Research Grants |