Paternal exposure to aluminum, acidity, and temperature affect fatty acid seminal profile, embryonic and larval development of Astyanax altiparanae

Author(s)

Silva Pinheiro, Joao Paulo ^[1] ; Lima, Jennifer ^[1] ; de Assis, Cecilia Bertacini ^[1] ; Branco, Giovana Souza ^[1] ; Gomes, Aline Dal'Olio ^[1] ; Moreira, Renata Guimaraes ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Lab Metab & Reprod Aquat Organisms LAMEROA, Matao St, 14 Lane, 101, Room 220, Cidade Univ, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Chemosphere; v. 266, MAR 2021.
• Web of Science Citations: 2

Abstract

We investigated the effects of water acidity, temperature, and aluminum (Al) on the fatty acid (FA) seminal profile, reproductive parameters (fertilization and hatching) and embryonic development of Astyanax altiparanae. We treated males with different experimental treatments, corresponding to the combination of water temperature (20 degrees C; 25 degrees C), pH (neutral - 7.0; acidic - 5.5), and the absence or presence of Al (0.5 mg L-1). After 96 h, we analyzed the FA profile of semen and performed artificial fertilization in activating medium with neutral pH or activating medium in the same experimental conditions of the males (neutral pH, acidic pH, and Al) to evaluate fertilization and hatching rates and to monitor embryonic development. Polyunsaturated FA percentage decreased in semen of fish from the neutral group, while monounsaturated FA increased in all groups maintained at 20 degrees C compared to 25 degrees C. Aluminum exposure decreased the percentage of C20:4n6 and increased the percentage of C22:5n3 at 20 degrees C. Males exposed to acidic pH and Al showed lower fertilization and hatching rates, as well as increased mortality of embryos and larvae. Moreover, Al favoured a higher percentage of abnormal larvae. Fertilization in Al activating medium harmed the embryos and larvae since fertilization and hatching rates decreased. Finally, temperature influenced fertilization time, hatching rate, and the morphology of embryos and larvae. Males exposed to Al had lower fertilizing capacity, which negatively affected the embryonic development of the species. Furthermore, Al activating medium reduced the number of fertilized oocytes, hatched embryos, and normal larvae. All events were temperature dependent. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
• Grantee: Carlos Arturo Navas Iannini
• Support Opportunities: Research Program on Global Climate Change - Thematic Grants
• FAPESP's process: 16/08770-8 - Evaluation of the semen quality, mutagenicity and genotoxicity in Astyanax altiparanae (Teleostei, Characidae) exposed to aluminum in different water temperatures
• Grantee: João Paulo Silva Pinheiro
• Support Opportunities: Scholarships in Brazil - Doctorate