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Evaluation of the semen quality, mutagenicity and genotoxicity in Astyanax altiparanae (Teleostei, Characidae) exposed to aluminum in different water temperatures

Grant number: 16/08770-8
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2017
Effective date (End): January 18, 2021
Field of knowledge:Biological Sciences - Zoology - Physiology of Recent Groups
Principal Investigator:Renata Guimarães Moreira Whitton
Grantee:João Paulo Silva Pinheiro
Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach, AP.PFPMCG.TEM
Associated scholarship(s):19/23816-2 - Effect of the plasticizer bisphenol A (BPA) on the thyroid axis of adult teleost fish, BE.EP.DR   18/10790-2 - Variation in the bioavailability and biological responses to drugs in fish, BE.EP.DR

Abstract

Anthropic actions on the environment, as the introduction of metals in water can cause genotoxic and cytotoxic effects on animals, and affect physiological processes such as reproduction. The toxicity of these metals, including aluminum (Al) can be potentiated when in synergy with high temperatures, influencing fish health and the quality of gametes, threatening the success of the reproduction. The hypothesis of this proposal is that Al, in synergy with the high temperature, can affect the seminal plasma and hence freezability of spermatozoa. The effects of exposure to Al will be assessed at different water temperature, on cytogenetic patterns, sperm characteristics, protein profile of seminal plasma and semen freezeability of Astyanax altiparanae. Sexually mature male A. altiparanae will be subdivided into nine groups: six groups without addition of Al, in acidic or neutral water pH in three different temperatures, 20, 25 and 30°C respectively, and the other three groups containing Al in the water (0.5 mg.L-1) at acidic pH, in the same temperatures described. The study will be carried out in two stages: 1) the animals exposed to the above conditions for 24 hours; and 2) the animals exposed for 96 hours; after each of these times, all animals from the tank will be sampled (n = 8) to run the toxicity tests, semen analysis and protein profile of seminal plasma. The blood will be collected to perform the micronucleus test and the comet assay. For seminal analysis, the animals will be induced to spermiation and subsequently the semen will be collected to analyze the osmolarity, pH, volume, morphology, kinetics, concentration, comet assay, micronucleus test, sperm ultrastructure and protein profile of the seminal plasma. The semen will be cryopreserved and semen quality parameters will be re-evaluated after thawing. Al content will be measured in gonads and semen. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA PINHEIRO, JOAO PAULO; DE ASSIS, CECILIA BERTACINI; SANCHES, EDUARDO ANTONIO; MOREIRA, RENATA GUIMARAES. Aluminum, at an environmental concentration, associated with acidic pH and high water temperature, causes impairment of sperm quality in the freshwater teleost Astyanax altiparanae (Teleostei: Characidae). Environmental Pollution, v. 262, . (14/16320-7, 16/08770-8)
SILVA PINHEIRO, JOAO PAULO; DE ASSIS, CECILIA BERTACINI; MUNOZ-PENUELA, MARCELA; BARBOSA JUNIOR, FERNANDO; CORREIA, TIAGO GABRIEL; MOREIRA, RENATA GUIMARAES. Water temperature and acid pH influence the cytotoxic and genotoxic effects of aluminum in the freshwater teleost Astyanax altiparanae (Teleostei: Characidae). Chemosphere, v. 220, p. 266-274, . (14/16320-7, 16/08770-8)
PINHEIRO, JOAO PAULO S.; WINDSOR, FREDRIC M.; WILSON, ROD W.; TYLER, CHARLES R.. Global variation in freshwater physico-chemistry and its influence on chemical toxicity in aquatic wildlife. BIOLOGICAL REVIEWS, v. 96, n. 4, p. 1528-1546, . (14/16320-7, 16/08770-8, 18/10790-2)
SILVA PINHEIRO, JOAO PAULO; LIMA, JENNIFER; DE ASSIS, CECILIA BERTACINI; BRANCO, GIOVANA SOUZA; GOMES, ALINE DAL'OLIO; MOREIRA, RENATA GUIMARAES. Paternal exposure to aluminum, acidity, and temperature affect fatty acid seminal profile, embryonic and larval development of Astyanax altiparanae. Chemosphere, v. 266, . (14/16320-7, 16/08770-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PINHEIRO, João Paulo Silva. Influence of aluminum and acid pH, associated with temperature variations, on seminal quality and reproductive variables of Astyanax altiparanae (Teleostei: Characidae). 2021. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.

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