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Variation in the bioavailability and biological responses to drugs in fish

Grant number: 18/10790-2
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 01, 2018
Effective date (End): March 01, 2019
Field of knowledge:Biological Sciences - Zoology - Physiology of Recent Groups
Principal Investigator:Renata Guimarães Moreira Whitton
Grantee:João Paulo Silva Pinheiro
Supervisor abroad: Charles Rupert Tyler
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : University of Exeter, Exeter, England  
Associated to the scholarship:16/08770-8 - Evaluation of the semen quality, mutagenicity and genotoxicity in Astyanax altiparanae (Teleostei, Characidae) exposed to aluminum in different water temperatures, BP.DR

Abstract

Improved understanding of the bioavailability of drugs in fish and their associated biological effects are needed to better assess the potential threat they may pose to wildlife. This project will undertake studies with selected drugs (antidepressants and non steroidal anti-inflammatory drugs) to establish how environmental conditions (water chemistry, specifically calcium concentrations) affects their uptake and associated biological responses in fish. The work will be conducted with zebrafish Danio rerio as the experimental model. The project will also apply a highly novel experimental approach, centred on the use of a transgenic zebrafish with a genetically-encoded pan neuronal Ca2+ sensor and cutting edge imaging, to establish how water chemistry affects the uptake of antidepressant drugs into the brain and identify the neural circuits affected by these exposures. As time allows for, the project will also apply appropriate bioassays to target specific behaviours, and assess how the water chemistry (calcium concentrations) modulated the behaviour effects and thresholds for these effects for antidepressants. The project will provide extensive training in leading experimental research methods and imaging technologies.