The Influence of Some Axial Ligands on Ruthenium-Phthalocyanine Complexes: Chemical, Photochemical, and Photobiological Properties

Author(s)

Martins, Tassia Joi ^{[1, 2]} ; Negri, Laisa Bonafim ^{[3, 4, 5]} ; Pernomian, Laena ^{[6, 5]} ; Faial, Kelson do Carmo Freitas ^[7] ; Xue, Congcong ^[1] ; Akhimie, Regina N. ^[1] ; Hamblin, Michael R. ^[8] ; Turro, Claudia ^[1] ; da Silva, Roberto S. ^{[3, 4, 5, 2]}

Affiliation

^[1] Ohio State Univ, Dept Chem & Biochem, Columbus, OH 43210 - USA

^[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, Ribeirao Preto - Brazil

^[3] Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 - USA

^[4] Harvard Med Sch, Dept Dermatol, Boston, MA 02115 - USA

^[5] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, Ribeirao Preto - Brazil

^[6] Univ Sao Paulo, Dept Pharmacol, Sch Med Ribeirao Preto, Ribeirao Preto - Brazil

^[7] Evandro Chagas Inst SAMAM IEC, Environm Sect, Ananindeua - Brazil

^[8] Univ Johannesburg, Laser Res Ctr, Fac Hlth Sci, Johannesburg - South Africa

• Document type: Journal article
• Source: FRONTIERS IN MOLECULAR BIOSCIENCES; v. 7, JAN 12 2021.
• Web of Science Citations: 0

Abstract

This work presents a new procedure to synthesize ruthenium-phthalocyanine complexes and uses diverse spectroscopic techniques to characterize trans-{[}RuCl(Pc)DMSO] (I) (Pc = phthalocyanine) and trans-{[}Ru(Pc)(4-ampy)(2)] (II) (4-ampy = 4-aminopyridine). The triplet excited-state lifetimes of (I) measured by nanosecond transient absorption showed that two processes occurred, one around 15 ns and the other around 3.8 mu s. Axial ligands seemed to affect the singlet oxygen quantum yield. Yields of 0.62 and 0.14 were achieved for (I) and (II), respectively. The lower value obtained for (II) probably resulted from secondary reactions of singlet oxygen in the presence of the ruthenium complex. We also investigate how axial ligands in the ruthenium-phthalocyanine complexes affect their photo-bioactivity in B16F10 murine melanoma cells. In the case of (I) at 1 mu mol/L, photosensitization with 5.95 J/cm(2) provided B16F10 cell viability of 6%, showing that (I) was more active than (II) at the same concentration. Furthermore, (II) was detected intracellularly in B16F10 cell extracts. The behavior of the evaluated ruthenium-phthalocyanine complexes point to the potential use of (I) as a metal-based drug in clinical therapy. Changes in axial ligands can modulate the photosensitizer activity of the ruthenium phthalocyanine complexes. (AU)

• FAPESP's process: 19/19448-8 - Effect of photobiomodulation as synergistic addendum on photocytotoxicity of ruthenium-phthalocyanine compounds as nitric oxide and singlet oxygen producers: in vitro biochemical evaluation in two- and three-dimensional (3D) tumor cell lines
• Grantee: Roberto Santana da Silva
• Support Opportunities: Regular Research Grants