Ruthenium nitrosil complex as producer of nitric oxide and singlet oxygen: FOTOBIOLOGICAL studies, BICHEMICAL and their potential therapeutical aplication

• Grant number: 14/03964-3
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): June 01, 2014
• Effective date (End): August 31, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Principal Investigator: Andréia Machado Leopoldino
• Grantee: Fernando Postalli Rodrigues
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 14/25071-0 - Assessment of metallocompounds using synchrotron FTIR spectromicroscopy and measurements on living cells, BE.EP.PD

Abstract

Elements supporting this project: (i) nitric oxide (NO) is a biological messenger of physiological and anticancer processes, (ii) its functions depend on its concentration and site of action, (iii) the use of NO donos compounds in clinical treatments such as photodynamic therapy (PDT) reach systems capable of releasing NO in a controlled way on biological systems, (iv) ruthenium- nitrosyl complex may be thermodynamically stable, but photochemically active, (v) the NO associated the release of singlet oxygen (1O2), increases the production of reactive oxygen species (ROS) and nitrogen (ERONs), (vi) both reactive species are involved in the modulation of cell death , eg apoptosis, (vii) species produce both NO and 1O2 photodynamic therapy against câncer.This project proposes the study of compounds that absorb radiation in the region of therapeutic window and which provide photoinduced electron transfer, in the presence of nitrosyl ruthenium complexes, can produce NO. Based on this the goal of this project is the study of energy transfer between Quantum Dots and [ (cp -R ) Runo (NO2) ] complex trans - ( PC -R is modified phthalocyanine ) and the type [ RuN4L (NO)]n+ and or [ RuN4L (NO2) ](1 -n +) (where N is nitrogen ligands and L can be pyridine , 4 - picoline and 4 - acetylpyridine), producing agents of reactive oxygen species ( ROS ) and nitrogen ( ERONs ) by light irradiation in the region of 500 nm to 700 nm which induce cell death in tumors.It will assess biochemical mechanisms related to interaction ruthenium complex as well as cell identification studies, activation and regulation of the signaling pathways of cell death. To this end, the following analysis tools will be used: proteomics, western blotting, confocal microscopy, flow cytometry as well as gene manipulation techniques, i.e., silencing / deletion of genes of interest. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, FERNANDO POSTALLI; MACEDO, LUCYANO J. A.; MAXIMO, LEANDRO N. C.; SALES, FERNANDA C. P. F.; DA SILVA, ROBERTO S.; CRESPILHO, FRANK N.. Real-time redox monitoring of a nitrosyl ruthenium complex acting as NO-donor agent in a single A549 cancer cell with multiplex Fourier-transform infrared microscopy. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 96, p. 29-34, MAR 1 2020. (13/07829-0, 16/12707-0, 15/16672-3, 13/14262-7, 17/20493-2, 14/03964-3)